1.3.2 sow disease sow endometritis: sow endometritis is the main cause of sow infertility. Inflammatory secretion of uterus hinders the movement and survival of sperm, making it impossible for sperm to reach the fertilization site; Salpingitis or edema, pyorrhea and ovarian adhesion can all cause tubal atresia and infertility. Some sows are infected with germs due to irregular operation during insemination, and the breeder brings germs into the uterus of sows, or the uterus is infected with germs during estrus, causing metritis. Some sows are often infertile due to reproductive diseases, and breeders should know the situation in time. Such sows are not suitable for breeding and should be eliminated as soon as possible. Reproductive disorders of sows: such as porcine highly pathogenic blue ear disease, classical swine fever, porcine pseudorabies, porcine parvovirus disease, etc. There are many factors that lead to the change of heart of sows after mating. In order to reduce the occurrence of back feeling, comprehensive measures must be taken, starting from the technical level of breeders, disease control, feeding management, environmental control and so on. 2. 1 semen quality is the basic standard for using semen: the dosage of semen is 80 ~ 100 ml, each head contains more than 3 billion effective sperm, and the sperm motility after dilution is ≥0.7. 2. 1.65438+
2. 1.2 Reasonable preservation
After the semen is stored at room temperature (25℃) of 1 ~ 2h, it can be stored in the incubator of 17℃, or it can be stored directly in the incubator of 17℃ with a towel wrapped tightly. The short-acting diluent can be stored for 3 days, the medium-acting diluent can be stored for 4-6 days, and the constant-acting diluent can be stored for 7-9 days. No matter what diluent is used to store semen, it should be used up as soon as possible. Gently rotate once every 12 h to prevent sperm from dying due to precipitation. Pay close attention to the temperature change of the incubator, and try to reduce the number of times of opening and closing the incubator. 2. 1.3 The transportation of shock-proof semen should be placed in a device with good heat preservation. In summer when the temperature is high, ice cubes should be added to the double-layer foam box, and then the semen should be transported to prevent overheating and excessive dead sperm. In cold season, cotton with good thermal insulation effect should be used to keep the temperature at 16 ~ 18℃. Strong vibration should also be avoided during sperm transportation. 2. 1.4 Sperm motility needs microscopic examination. Semen quality should be checked, including semen volume, color, smell, sperm density, vitality, abnormal rate, etc. To ensure the quality of semen is qualified. Sperm motility should be checked after sperm collection, and fresh sperm motility should be ≥0.7. When checking sperm motility, glass slides and cover glasses should be preheated at 37℃. After dilution, it is required to stand for a period of time before sperm motility examination. If the vitality changes little before and after dilution, it can be stored in separate packages. If the vitality is obviously reduced, it should not be used. It is particularly necessary to check sperm motility before mating. Take the semen out of the incubator at 17℃, shake it gently, put 1 drop on the preheated glass slide with a sterilized dropper, put it in the incubator at 37℃ for a period of time, and check the sperm vitality with a microscope. Sperm viability ≥0.7 can only be used.
2. 1.5 The instrument needs disinfection. The laboratory should be neat, clean and hygienic. All utensils should be cleaned with detergent or washing powder, washed with distilled water, and dried at 60℃ (the drying temperature of glassware can be higher than 100℃), and then the opening of the utensils should be wrapped with tin foil, and the glassware should be sterilized with 180℃ 1h in dry heat, and the non-heat-resistant utensils and appliances should be used with1h. 2.2 Practice internal strength and master excellent mating skills. 2.2. 1 Identifying estrus and mating in time is the key. Estrus identification is the premise of timely breeding and the main link to improve pregnancy rate. The key is to accurately estimate the ovulation time. The estrus cycle of sows can be divided into pre-estrus, estrus, post-estrus and inter-estrus. Only in estrus can sows make boars get up or stand up, which can be used as an indicator of timely insemination of sows. The first insemination was carried out at 8 ~ 12h after oestrus sows showed static reflex, and then the second or third insemination was carried out every 8 ~ 12h. 2.2.2 Mastering insemination technology is a means of breeding technology, which is directly related to the conception rate. Anyone engaged in this work must strictly follow the insemination procedure in practice. The procedure of insemination is as follows: ① Disinfect and clean the hands of insemination personnel. ② Wash the vulva, tail root and buttocks of sow, then soak the towel with warm water and dry the vulva. ③ Take out the sterilized vas deferens from the sealed bag. Don't touch the first 2/3 part of vas deferens, and apply lubricant to the front end. ④ Insert the vas deferens upward at a 45-degree angle into the sow reproductive tract. When you feel resistance, slowly rotate counterclockwise and move back and forth until you feel that the front end of the vas deferens is locked (gently pull back), and confirm that it is locked by the cervix.
⑤ Take out the qualified semen from the semen storage box, and confirm the boar breed and spike number.
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Attachment: sow reproduction
The individual development of pigs originated from fertilized eggs. In order to breed a large number of high-quality piglets, sows must be in normal estrus and excrete a large number of high-quality eggs in addition to obtaining a large number of high-quality semen.
Spare sows can promote sow estrus, including contact with the opposite sex, feeding management, feeding complete feed and paying attention to energy protein balance.
Attention should be paid to the breeding of multiparous sows, and comprehensive diets should be provided during the breeding period to maintain the physical condition of sows. If sows are overweight, they will not be in estrus, ovulate less, and their eggs will be weak and empty. Too thin can also cause postpartum estrus delay and other undesirable phenomena.
In order to breed sows, timely breeding is needed. Sows ovulate 24-36h after estrus, and the ovulation lasts 10- 15h. Eggs can be fertilized in fallopian tubes for 8- 12h, sperm can reach fallopian tubes in reproductive tract for 2-3h, and sperm can survive for 20-30h. Therefore, the suitable mating time is 2-3 hours after ovulation, that is, 20-30 hours after estrus. In recent years, the research tends to be early mating, that is, the first mating is carried out 24 hours after estrus and the second mating is carried out 18-24 hours after the first mating. If the sow is still willing to breed 18-24 hours after the second mating, it can be mated for the third time.
Sows are not in estrus, and the production conditions are not good, so they may not be able to breed in time.