Summary: This paper summarizes the research results and progress of chemical constituents of traditional Chinese medicine compound, including qualitative and quantitative analysis of effective chemical constituents, extraction, separation and identification of chemical constituents of the whole prescription, and pharmacological tracking of active parts and effective components of the compound. Compound prescription of traditional Chinese medicine is the main form of clinical application of traditional Chinese medicine, and the chemical components in compound prescription are the material basis for traditional Chinese medicine to exert its efficacy. The study of chemical constituents of compound prescription is of great significance to clarify the theory of traditional Chinese medicine, reveal the compatibility law and mechanism of traditional Chinese medicine, optimize preparation technology, formulate quality control standards, realize the modernization of traditional Chinese medicine and go to the international market. In this paper, the research on chemical constituents of traditional Chinese medicine compound is reviewed for reference. The research methods and approaches of 1 Up to now, the research on the chemical components of traditional Chinese medicine compound is still in the exploratory stage in terms of ideas, techniques and methods, and many authors have put forward some meaningful views and ideas, such as the ternary design scheme for systematic separation and identification of chemical components of traditional Chinese medicine compound [1]. The shotgun theory put forward by Xue Yan and others [2] and the natural combinatorial chemical library and multi-target mechanism put forward by Zhou Jun and others [3]. It has certain enlightenment and reference value for how to study the chemical constituents of traditional Chinese medicine compound. At present, the research methods and approaches of chemical components of traditional Chinese medicine compound can be summarized as the following three aspects: 1) Taking the effective components of single medicine as indicators, the whole prescription preparation is qualitative and quantitative. 2) Systematic extraction, separation and identification of chemical components of the whole prescription were carried out by phytochemical methods. 3) Tracking the active parts and components of the compound according to the efficacy. 2. Qualitative and quantitative determination of the main effective chemical components of a single drug as a marker substance, qualitative and quantitative determination of the index substances (components) in the compound, and the influence of various separation and analysis techniques on the compatibility of each drug and each single drug preparation, preparation conditions (particle size, decocting apparatus, water addition, soaking time, decocting time, decocting times, heating temperature, decocting separately, decocting first and then decocting, etc.). ), the effects of preparation methods (single decoction, separate decoction and combined decoction), compatibility and dosage form on the quality and quantity of index substances (components) were also discussed. This kind of research work has been carried out and some meaningful results have been achieved. Siwutang is composed of Radix Angelicae Sinensis, Radix Rehmanniae, Radix Paeoniae Alba and Rhizoma Chuanxiong. Yuan Jiurong et al. [4] determined the contents of ferulic acid, 8 kinds of trace elements, 65,438+07 kinds of amino acids and water-soluble decoction in single decoction, separate decoction and combined decoction of Siwutang by various analytical methods. The results show that there is solubilization between the components when they are co-fried under heating conditions. Zhong Lixian et al [5] determined and compared the contents of ephedrine in Xiaoqinglong Decoction (composed of Herba Ephedrae, Ramulus Cinnamomi, Radix Paeoniae Alba, Radix Glycyrrhizae, etc.). The results show that the content of ephedrine in the combined decoction is the lowest, which is caused by the precipitation of glycyrrhizic acid and ephedrine, but there is no significant difference in the efficacy between the combined decoction and the separate decoction, which shows that glycyrrhizic acid and ephedrine are still effective in the body after oral administration, so they are effective in the processing of Chinese medicine polyjuice potion. Sini decoction consists of aconite, licorice and dried ginger. Zhang Yu et al. [6] made qualitative and quantitative analysis on the main effective components of Radix Aconiti Lateralis and Radix Glycyrrhizae, Radix Aconiti Lateralis and Rhizoma Zingiberis, and the results showed that the content of toxic aconitine components increased when Radix Aconiti Lateralis and Rhizoma Zingiberis were compatible. However, when aconite is mixed with licorice, the content of aconitine decreases, which shows that the theory of "aconite is not hot and dried ginger, but licorice is slow" in traditional Chinese medicine has certain scientific basis. Liuwei Dihuang Decoction is a famous prescription for nourishing yin. Yan Yongqing and others [7 ~ 9] made a preliminary analysis of its chemical constituents. The results show that the quality and quantity of chemical components in the same prescription are different due to different preparation processes. The chemical composition of compound prescription is not equal to the simple addition of the chemical composition of each single drug; There are more kinds of chemical components in the combined decoction than in the separate decoction. Zhu Yongxin et al. [10] found that the contents of ginsenoside Rg3 and Rh 1 in Shengmaisan decoction were significantly higher than those in single-component ginseng decoction, thus inferring that the hydrolysis and transformation of ginsenoside occurred during the heating decoction, and as a result, Rg3 and Rh 1, which were originally trace components in single-component medicine, became the main components in the compound. Yan Yongqing and others [7] found that the content of total ginsenoside in the combined decoction of ginseng, Ophiopogon japonicus and Schisandra chinensis was lower than that in the separate decoction, but in the observation of hemodynamics, myocardial effect and clinical efficacy, the combined decoction was better than the separate decoction. Therefore, it is speculated that ginsenoside Rg3 and Rh 1 may be the active components of this prescription in some pharmacological actions and clinical effects. Wei Huifen et al. [1 1] compared the chemical constituents of Xiao Banxia plus Fuling Decoction and its prescriptions. The results showed that the alkaloid content in the compound prescription was lower than that in the single drug of Pinellia ternata, but the amino acid content was higher than that in each single drug. It is considered that the high content of amino acids is beneficial to the stomach-regulating and antiemetic effects of prescriptions. Wuren liquid is a bactericide extracted from hawthorn seeds and other traditional Chinese medicines. Tu Jiasheng et al. [12] analyzed its chemical constituents by GC/MS, and found that it was rich in effective components with antibacterial effect, such as phenols, benzoic acids and fatty acids, and the relative contents of various effective components were calculated by area normalization method. Fructus Aurantii Immaturus Pill consists of Fructus Aurantii Immaturus and Atractylodis Rhizoma. Luo Shangfeng et al. [13] used GC/MS method to determine the dynamic changes of the contents of four effective components: atractylone, atractylone, hydroxyl atractylone and dehydrated hydroxyl atractylone. The results showed that atractylone in Atractylodes macrocephala could be oxidized to atractylone and hydroxyl atractylone during processing, but it could be reduced to atractylone after combining with Fructus Aurantii Immaturus, and this was discussed. 3. Extraction, separation and identification of chemical components by phytochemical methods. Taking the compound prescription of traditional Chinese medicine as a whole, the chemical components of the whole prescription are systematically extracted, separated, purified and identified by phytochemical methods, so that we can comprehensively analyze what the chemical components of the compound are, what is the difference between it and a single drug, and whether there are new compounds. At present, there are few reports about this research work. 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