Principle of second generation sequencing:
A method called Sanger method is used to decompose the DNA molecular chain and then sequence it, so that a complete DNA chain can be obtained and the DNA sequence can be determined. The advantages of the second generation sequencing technology are fast, accurate and efficient, and it can simultaneously determine a large number of samples.
Second generation sequencing applications:
The application of the second generation sequencing technology can be summarized as genome sequencing, transcriptome sequencing, methylation sequencing, resequencing and protein omics. Among them, genome sequencing can be used to study genetics, disease genes, gene expression, biodiversity and in-depth study of genome structure and function.
Introduction to the second generation sequencing;
The second generation sequencing technology is a sequencing technology, which can quickly and accurately determine the sequence of DNA, RNA, protein and other substances in a short time, and it is one of the important technologies in life science research in 2 1 century.