(4) Micro-counting method must use a blood cell counting plate to count the cells in the grid to determine the optimal concentration.
2. According to the analysis topic, the proliferation of cancer cells can only be completed in "appropriate temperature" or "incubator". When the test tube stands, the solution inside will precipitate, and the number of cells per milliliter obtained when absorbing the culture solution is inaccurate, so it should be shaken well before absorption.
3. The experimental design should follow the control principle and the univariate principle. It can be seen from the experimental data in the figure that dichlorodiethylamine has inhibitory effect on the proliferation of cancer cells, and within a certain range, with the increase of dichlorodiethylamine concentration, the inhibitory effect is gradually enhanced.
So the answer is:
1, (2) equal amount of normal saline (4) blood cell counting plate
2.① In the refrigerator, it should be changed to a suitable temperature or a constant temperature box. ② In the five static test tubes, the appropriate amount of culture solution should be changed to "After shaking, absorb the appropriate amount of culture solution from the five test tubes".
3, (1) control, univariate?
(2) Dichlorodiethylamine can inhibit the proliferation of cancer cells? In a certain range, with the increase of dichlorodiethylamine concentration, the inhibitory effect gradually increased.