Tswett applied chemical methods to study cell physiology. 1900, he discovered two kinds of chlorophyll in leaves: chlorophyll a and chlorophyll b, and later discovered chlorophyll c, and isolated pure chlorophyll. His greatest contribution is the invention of chromatography, which is an extremely important experimental method in analytical chemistry and organic chemistry. His first paper on chromatography was published in the Journal of Biology in Warsaw, 1903. Papers from 1906 to 19 10 were published in the German Journal of Botany. In these papers, he described in detail the separation of carotene, chlorophyll and lutein with his own chromatographic analysis instrument. Because his paper was published in a little-known journal, it did not attract the attention of the chemical community at that time. It was not until 193 1 year that R. Kuhn discovered the importance of chromatography invented by Tswett, and this method was widely applied.