The key theory and technical system of liver surgery were established.

The study of liver anatomy began from 1958. Based on the establishment of perfusion corrosion model of human liver, detailed observation and surgical practice, the anatomical theory of "five leaves and four segments" was creatively put forward. In order to solve the important problem of bleeding in liver surgery, on the basis of animal experiments and clinical exploration, a liver hemostasis technique of "intermittent hilar occlusion at room temperature" was established. In order to master the changes of biochemical metabolism after liver surgery and reduce the operative mortality, through clinical and liver biochemical research, the "biochemical metabolism law after normal and liver cirrhosis surgery" was found, and a new strategy to correct the common fatal biochemical metabolism disorder after liver cancer surgery was put forward. In order to further expand the indications of liver surgery and improve the level of liver surgical treatment, he took the lead in successfully performing a series of landmark operations represented by middle lobe resection. On the basis of the above work, the unique key theory and technology of liver surgery were established, and the subject system of liver surgery in China was established, which gradually developed and expanded.

It opens up a new field of basic and clinical research of liver cancer.

He put forward the concept of "two-stage operation" in view of the late, huge and unresectable characteristics of liver cancer when it was discovered, that is, comprehensive treatment was carried out for huge liver cancer first, and then surgical resection was carried out after the tumor shrank, which opened up a new treatment way for the treatment of advanced liver cancer; In view of the recurrence of liver cancer after operation, but the lack of effective treatment, the viewpoint of "reoperation of recurrent liver cancer" was put forward for the first time, which significantly prolonged the survival time of patients with liver cancer. In view of the characteristics that liver cancer combined with cirrhosis in China is easy to lead to postoperative liver failure, a local radical treatment strategy for liver cancer is proposed to organically unify the efficacy and safety of liver cancer surgery. The above research improved the 5-year survival rate of liver cancer from 16.0% in 1960s and 1970s to 30.6% in 1980s and 48.6% since 1990s, which continuously enriched and developed the cause of liver surgery in China. In order to improve the scientific research level of liver surgery in China and further develop the cause of liver surgery, Academician Wu established the world's largest professional institute of liver surgery, led a series of internationally advanced basic research work, developed two tumor vaccines, cell fusion and bispecific monoclonal antibody modification, and invented a proliferative virus vector carrying anti-cancer genes. The research results were published in academic journals such as Science, Natural Medicine, Hepatology, Oncogenes and Cancer Research.

Established the world's largest liver disease research and diagnosis and treatment center, and trained a large number of high-level professionals.

Establish Wu Hepatobiliary Surgery Medical Fund to reward outstanding talents and innovative research who have made outstanding contributions to the cause of hepatobiliary surgery in China; A large number of senior professionals have been trained. Through the joint efforts of him and his colleagues, he promoted the development of liver surgery at home and abroad. Most theories and techniques of surgical treatment of liver cancer originated in China, which made China in a leading position in international research, diagnosis and treatment in this field. In order to lay the foundation of liver surgery, Wu studied liver anatomy from 1958, and creatively put forward the anatomical theory of "five leaves and four segments" on the basis of establishing the perfusion corrosion model of human liver and making detailed observation and surgical practice. In order to solve the important problem of bleeding in liver surgery, on the basis of animal experiments and clinical exploration, a liver hemostasis technique of "intermittent hilar occlusion at room temperature" was established. In order to master the changes of biochemical metabolism after liver surgery and reduce the operative mortality, through clinical and liver biochemical research, the "biochemical metabolism law after normal and liver cirrhosis surgery" was found, and a new strategy to correct the common fatal biochemical metabolism disorder after liver cancer surgery was put forward. In order to further expand the indications of liver surgery and improve the level of liver surgical treatment, he took the lead in successfully performing a series of landmark operations represented by middle lobe resection. On the basis of the above work, the unique key theory and technology of liver surgery were established, and the subject system of liver surgery in China was established, which gradually developed and expanded.

In 1950s, he first put forward a new viewpoint of liver anatomy with five leaves and four segments in China. In the 1960s, intermittent hilar occlusion was pioneered, and it was the first to break through the forbidden area of mid-term surgery. In 1970s, a complete system of early diagnosis and treatment of hepatic cavernous hemangioma and small hepatocellular carcinoma was established. Hepatic artery ligation and hepatic artery embolization were used to treat advanced hepatocellular carcinoma in the early stage. In 1980s, normal temperature bloodless hepatectomy, re-resection of recurrent liver cancer and second-stage operation of liver cancer were established. In 1990s, great progress was made in gene immunotherapy and liver transplantation for advanced liver cancer, and laparoscopic hepatectomy and hepatic artery ligation were carried out for the first time. Over the past 40 years, * * * has performed more than 8,000 liver cancer operations, with a 5-year survival rate of 38. 1%. More than 1,000 cases of small hepatocellular carcinoma (less than 5 cm)/kloc-0 were treated by surgery. The 5-year survival rate was 79.8% (85.3% of them were small hepatocellular carcinoma (less than 3 cm), and the longest survival time was 36 years.

In 1960s, he initiated a simple and safe new treatment of liver surgery-transanal intermittent blocking hepatectomy, and broke through the forbidden area of the liver, successfully performing middle lobe resection. Wu has trained a large number of high-level professionals, and the scale of his discipline has developed from a "three-person research group" to the current third-class first-class specialized hospital and hepatobiliary surgery research institute, becoming the world's largest hepatobiliary disease diagnosis and treatment center and scientific research base; Establish Wu Hepatobiliary Surgery Medical Fund to reward outstanding talents and innovative research who have made outstanding contributions to the cause of hepatobiliary surgery in China; A large number of senior professionals have been trained. Through the joint efforts of him and his colleagues, he promoted the development of liver surgery at home and abroad. Most theories and techniques of surgical treatment of liver cancer originated in China, which made China in a leading position in international research, diagnosis and treatment in this field. Since 1978, under his guidance, the hospital has trained 23 postdoctoral students, 67 doctoral students and 85 master students. Such as Wang Hongyang, deputy director of the Institute of Oriental Hepatobiliary Surgery, Second Military Medical University.