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Fang academic paper
1. Xiaogang Feng, Wang Xinan, Zhang Xiaofeng, Wu Beibei, Hu, Pan, Cheng Shiyun, Fang. Pathogenic characteristics of Vibrio parahaemolyticus from clinical and seafood sources. International Journal of Food Microbiology, 2008, accepted.

2. Wang Shuna, Fang. Differential expression of tdh gene in Vibrio parahaemolyticus was detected by fluorescence quantitative PCR. Animal Husbandry and Veterinary, 2008, has been hired.

3. Wu Di, Zhao Huancan, Xie Ke Adikate, Fang. Pathogenicity of Streptococcus suis type 2 isolate to wild zebrafish. Advances in Animal Medicine, 2008, has been rented.

4. Liu Gang, Li Xiaoliang. Sequence analysis and expression of h gene of canine distemper virus. Animal Husbandry and Veterinary, 2008, has been hired.

5. Chen Jianshun, Ma Youzhi, Fang. Establishment and application of triple PCR for detection of Streptococcus suis type 2. China Veterinary Journal, 2008, included.

Xiaogang Feng, Pan, Zhang Xiaofeng, Cheng Shiyun, Meryl, Xu Chun, Wang Xinan, Fang. Cloning of Vibrio parahaemolyticus in seafood and clinical samples from a coastal province of China. Foodborne pathogens and diseases, 2008,5 (2):127-134.

7. Yang Zhenzhong, Shuai Jianbin, Dai, Fang. Investigation of porcine circovirus type 2 infection and phylogenetic analysis of ORF2 gene in Hangzhou, Zhejiang Province. Journal of Zhejiang University Series B, 2008,9 (2):148-153.

8. Jiang Xiaolin, Chen Jiansheng, Xu, Zhang Xiaofeng, Wang Xinan, Zhao Huicong, Xiaogang Feng, Fang. Virulence characteristics and genotype analysis of Listeria monocytogenes in food and processing environment in East China. International Journal of Food Microbiology, February, 20081(1): 53-59.

9. Shuai Jianbin, Li Xiaoling, Chen Ning, Chen Chunying, Fang. Characteristics and potential use of truncated PCV2 capsid protein and its polyclonal antibody in the diagnosis of PCV2 infection. Acta Microbiologica, 2008:489 1):85-90.

10. Shuai Jianbin, Wei Wei, Jiang Xiaoli, Li Xiaoling, Chen Ning, Zhang Fang. Localization of nuclear localization signal of porcine circovirus 1 open reading frame 2 protein. Journal of Biochemistry and Biophysics, 2008,40 (1): 71-77.

1 1. Chen Ning, Hu, Zhang, Shuai Jianbin, Fang. Genetic diversity of envelope glycoprotein E2 of classical swine fever virus: a new isolate from historical and vaccine strains. Veterinary Microbiology, 2007, 127:286-299.

12. Shuai Jianbin, Wei Wei, Li Xiaoling, Chen Ning, Zhang, Chen Xiaoyan, Fang. Genetic characteristics of porcine circovirus type 2 in high seroprevalence areas in southeastern China. Viral gene, 2007, 35:6 19-627.

13. Chen Jianshun, Jiang, Fang. Virulence factors and evolution of listeria. Journal of Microbiology, 2007,47 (4): 738-742.

14., Chen Yabo, Fang. Prokaryotic expression and antigenicity analysis of structural protein VP 1 of foot-and-mouth disease virus type O. Advances in Animal Medicine, 2007,28 (6):14-18.

15. Ying Weifang, Zhao Huancan, Qian Ya, Fang. Detection and drug sensitivity test of Streptococcus suis to be slaughtered in slaughterhouses in some areas of Zhejiang Province. Advances in Animal Medicine, 2007,28 (6): 24-26.

16. Pan Zijiang, Kambu, Shen Biao, Lou Gaoming, Fang. Phenotype and hemolysin-related genes analysis of Vibrio parahaemolyticus isolated from seafood and clinic. Acta Microbiologica, 2007,47 (3): 508-511.

17., Li Xiaoliang, Chen Jianshun, Fang. Expression of LMO 1847 gene of Listeria monocytogenes under different stress conditions. Animal Husbandry and Veterinary, 39(7):7- 10.

18. Chen Xiaoyan, Xu, Shuai Jianbin, Chen Jiansheng, Zhang, Fang. The surface protein of Lactobacillus Curvulus strain ZJ00 1 is responsible for the competitive rejection of Escherichia coli O 1 57:H7 and Salmonella typhimurium. International Journal of Food Microbiology, 2007,115 (3): 307-312.

19. Yang Zhongze, Habimi, Shuai Jianbin, Fang. Detection of PCV2 DNA by SYBR Green I-based quantitative PCR, Journal of Zhejiang University B, 2007,8 (3):162-169.

20. Jiang Lier, Ke Chunlin, Xu, Chen Jiansheng, Chen Xiaoyan, Chen Ning, Shuai Jianbin, Fang. Listeria monocytogenes mutant carrying Newcastle disease virus F gene and its in vitro expression and immunogenicity in chickens, Journal of Biochemistry and Biophysics, 2007, 39( 1):57-66.

2 1. Chen Yabo,,, Fang. Epitope analysis of HN protein of Newcastle disease virus and prokaryotic expression of domain gene. Chinese Journal of Preventive Veterinary Medicine, 2007,29 (1): 32-35.

22. Li Xiaoling, Shuai Jianbin and Fang *. Protective effect of egg yolk specific immunoglobulin on the resistance of crucian carp to Aeromonas hydrophila infection. Journal of Zhejiang University, Natural Science Edition, July 2006 (11): 922-928.

23. Yang Zhenzhong, Fang, Habib M. Quantitative PCR detection and preliminary results of RNA based on sybr green I. Journal of Veterinary Medicine B, 2006; 53( 10):46 1-467.

24. Li L, Fang, Li JR, Huang, Yu L. Protective immune response was induced in chickens by oral DNA vaccine of infectious bursal disease virus multi-protein gene delivered by attenuated Salmonella. Vaccine, 24, 2006 (33-34): 5919-5927.

25. John Dickimi, Chen Xiaoyan, Chen N, Fang *. Attenuated Salmonella typhimurium as a prokaryotic and eukaryotic expression vector, Journal of Zhejiang University (Agric. & Life Science. ,ed),2006,32(3):237-244。

26. Fang, Birgitte B, Siegumfeldt H. Leuconostoc Leuconostoc 40 10 causes matrix-related decrease of intracellular pH of Listeria monocytogenes, FEMS Microbiology Bulletin, 2006,258 (2): 208-213.

27. Jiang LL, Xu, Chen N, Shuai Jianbin, Fang. Analysis of virulence phenotype and molecular characteristics of a low pathogenic Listeria monocytogenes, Journal of Biochemistry and Biophysics, 2006,38 (4): 262-270.

28. Xu, Jiang, handsome and square. Construction and identification of recombinant attenuated listeria monocytogenes carrying foreign genes. Journal of Microbiology, 2006,46 (3): 445-450.

29. Feng Kebo, Cheng Xueyan, Zhou Xiaoyan, Shen Bo, He Xiaoling, Zhang Fang used double PCR to detect the prevalence of Vibrio parahaemolyticus in seafood and its processing environment. Journal of Food and Agricultural Sciences, 2006,86 (12):1871-1877.

30. Chen Yabo, Zhang, Fang. Prokaryotic expression and epitope analysis of Newcastle disease virus F protein domain gene. Journal of Zhejiang University (Agriculture and Life Sciences Edition) 2006,32 (1): 82-87.

3 1. Yang, Fang. Classical swine fever complicated with porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) infection. China Veterinary Journal, 2006,26 (3): 240-242.

32. Ceng Haiyan, Zhang Xiaofeng, Sun Zhi and Fang. Identification of Listeria monocytogenes in milk and milk processing environment by multiplex PCR. Journal of Food and Agricultural Sciences, 2006,86 (3): 367-371.

33. Yang, Sun and Fang. Hemolytic activity and adjuvant effect of astragaloside on immune response of mouse ovalbumin. Vaccine, 2005, 23 (44): 5 196-5203.

34. Yin, Qian Ya, Fang. Purification of IgY against Aeromonas hydrophila and its antibacterial activity in vitro. Journal of Zhejiang University (Agriculture and Life Sciences Edition), 2005,31(4): 503-506.

35. Dai Xianjun, Fang. Safety of oral somatostatin DNA vaccine for grass carp with attenuated Salmonella as carrier. China Veterinary Journal, 2005,25 (5): 466-469.

36. Dai Xianjun, Fang. Construction and stability of oral somatostatin DNA vaccine against attenuated Salmonella in grass carp. China Veterinary Journal, 2005,25 (4): 356-358.

37. Ma Youzhi, Dai Xianjun, Fang. Construction and identification of attenuated Salmonella expressing hemolysin gene of Streptococcus suis. China Veterinary Journal, 2005,25 (5): 478-480.

38. John Dickie, Jiang, Shuai and Fang. Stability of recombinant plasmid in attenuated salmonella and its influence on bacterial invasion. Journal of Microbiology, 2005,45 (5): 744-747.

He Xiaolong, Fang. Establishment of specific PCR method for Escherichia coli O 157:H7 in animal food. Chinese Veterinary Science and Technology, 2005,35 (8): 626-629.

40. Ke Chunli, Song Haohui, Jiang Xiaoling, Ceng Haiyan, Zhang Fang. Construction of recombinant listeria monocytogenes mutant expressing green fluorescent protein. Journal of Zhejiang University (Agricultural Edition). & Life Science. ,ed),2005,3 1(5): 638-644。

4 1. Jiang, Song, Chen Xiaoyan, Ke Chunlin, Xu, Chen, Fang. Identification of a mutant strain of Listeria monocytogenes expressing green fluorescent protein. Journal of Biochemistry and Biophysics, 2005,37 (1):19–24

42. Song HH, Wang, Zheng DX, Fang, Li Y, Liu, Niu, Qiu BS. A novel mucosal vaccine against foot-and-mouth disease virus induces protection in mice and pigs. Biotechnology Express, 2005,27 (21):1669-1674.

43. Song HH, Fang, Wang, etc. The antibody of foot-and-mouth disease virus was detected by using the protein purified from the codon-optimized composite epitope of foot-and-mouth disease virus expressed at high level in Escherichia coli. Biotechnology Bulletin, 26, 2004 (16):1277-1281

44. Jiang Zhongqi, Fang, Zhang,. Surveillance of drug resistance of Escherichia coli in weaning diarrhea of piglets in large-scale pig farms. Journal of Zhejiang University (Agriculture and Life Sciences Edition), 2004,30 (5): 567-571.

45., square,, stone. Comparative study on selective enrichment culture and isolation of listeria. China Public Health, 2004,20 (6): 718-719.

46. Ma Youzhi, Fang. Rapid detection of hemolysin gene of Streptococcus suis type 2 by PCR. Journal of Zhejiang University (Agriculture and Life Sciences Edition) 2004,30 (1): 78-82

47. Fang, Fan Yongjun, Xu Jian,,,. Expression of infectious bursal disease virus polyprotein gene in Vero cells with attenuated Salmonella as vector. Journal of Bioengineering, 2004,20 (3): 437-440.

48. Fang, Siegumfeldt H, Budde BB, Jakobsen M. Through fluorescence ratio imaging microscope analysis, osmotic stress caused the intracellular pH of Listeria monocytogenes to decrease. Application and technology. Environmental microbiology, 2004,70 (5): 3176-3179.

49. Song HH, Zhou L, Fang, Li Yong, etc. Efficient expression of codon-optimized composite epitope of foot-and-mouth disease virus and cholera toxin B subunit chimera in Hansenula polymorpha. Biochemistry and biophysics research newsletter, 2004,315 (1): 235-239.

50. Song Haohui, Li Yan, Fang, Geng, etc. Construction of expression vector of hansenula polymorpha. Biotechnology Express, 2003,25:1999-2006.

5 1. Xie, Fang, Lumiga,. Construction of eukaryotic expression vector of Xiaoshan chicken IL-2 gene and its expression in VERO cells. Journal of Zhejiang University, 2003,29 (6): 649-654.

52. Liang Xueya, Fang, Jiang. Safety, stability and immune effect of oral DNA vaccine against Newcastle disease in chickens. Journal of Bioengineering, 2003, 19 (1): 24-29.

Ma Youzhi, Fang, Ke Chunlin, Biological characteristics of Streptococcus suis type 2 JX isolate. China Veterinary Journal, 2003,23 (4): 326-328.

54. Ma Youzhi, Fang. Cloning and sequence analysis of hemolysin gene of Streptococcus suis type 2 JX02 strain. China Veterinary Journal, 2003,23 (5): 460-462.

55. Fang, Liang Xueya, Immunoprotective effect of DNA vaccine with attenuated Salmonella on Newcastle disease virus. China Veterinary Journal, 2003,23 (6): 527-529.

56. Fang, Jiang and Ke Chunlin. Identification of Listeria monocytogenes by multiplex polymerase chain reaction. China Journal of Laboratory Medicine, 2003,26 (2) 93.

57. Fang, Jiang and Du Huahua. Detection of Listeria monocytogenes in food by polymerase chain reaction. Chinese Journal of Preventive Medicine, 2003,37 (3):199.

58. Fang Weihuan. The construction of food safety standards and monitoring system should be in line with international standards as soon as possible. International academic trends, 2003, 1: 15- 18.

59. Cheng Jie and Fang. Research progress on immune mechanism of Listeria monocytogenes. China Journal of Zoonomatosis, 2003,19 (5):113-15.

Fang Fang and Liang Xueya. Expression of Newcastle disease virus F protein in eukaryotic cells with attenuated Salmonella as vector. Journal of Biochemistry and Biophysics, 2002,34 (4): 488-493.

6 1. Zhong, Weng Haibo, Fang. Preparation of protein samples for gel electrophoresis by sequential extraction. Journal of Zhejiang University, Science Edition. , 2002,3(5)606-6 10.

62. Song Houhui, Fang. Cloning and sequence analysis of hemolysin gene of Streptococcus suis ZJ strain. Chinese Journal of Preventive Veterinary Medicine, 2002,24 (2):103-105.

63. Liang Xueya and Fang. Advances in molecular biology of porcine reproductive and respiratory syndrome virus. China Veterinary Science and Technology.2001,31(8).14-17.

64. Song Houhui, Fang. Advances in molecular biology of main virulence factors of Clostridium perfringens. Progress in Preventive Veterinary Medicine, 200 1, 3 (1):13-16.

65. Fang WH, Almeida R.A. Effects of lactoferrin and milk on adhesion of Streptococcus lactis to bovine mammary epithelial cells. American Journal of Veterinary Research, 2000, 3:275.

66. Fang WH Identification of lactoferrin binding protein from mastitis of dairy cows causing Streptococcus lactis. FEMS microbiology letters, 1999, 176:9 1.

67. Almeida R.A. Adhesion and internalization of Streptococcus lactis to bovine mammary epithelial cells are mediated by proteoglycan, the host cell. FEMS medical newsletter, 1999, 177:3 13.

68. Fang WH Luther d.a. Protein expression of Streptococcus lactis in co-culture with bovine mammary epithelial cells. FEMS microorganisms. Letters, 1998, 166:237.

69. Fang WH Luther d.a. Almeida R.A. Oliver S.P. reduced the growth of Streptococcus lactis in the mammary glands of dairy cows attacked by the same mastitis pathogen. Journal of Veterinary Medicine B, 1998, 45:295.

70. Ali-Vehmas T., Vikerpuur M., Fang. Supplementing selenium to dairy cows enhanced the inflammatory response to intra-mammary infection and inhibited the growth of mastitis pathogens in whey. Journal of Veterinary Medicine A, 1997, 44:559.

7 1. square WH. Melissa v.&; Resazurin reduction as a function of respiratory burst of bovine polymorphonuclear leukocytes. American Journal of Veterinary Research,1997,58 (6): 601.

72. Fang WH A new fluorescence assay was used to evaluate the post-antibiotic effect on Staphylococcus aureus and Escherichia coli causing mastitis. Journal of Microbiological Methods,1996,26:151.

73. Fang WH Detection of Staphylococcus aureus and Escherichia coli in liquid culture medium by fluorescence quantitative method and its application in phagocytosis test. Journal of Apple Bacteriology, 1996, 80:577.

74.Koivunen A-L, Maisi P., Fang. & Inhibition of protease activity in tracheobronchial aspirate of horses with chronic obstructive pulmonary disease. American Journal of Veterinary Research,1996,57 (5): 603.

75. Marbe, Salonen, Fang. Aupic T, Jia lacasse M, klassen M. & Ampsandholm M. Distribution of BaytrilR in Breast after Intravenous and Intra-arterial Injection. Journal of Veterinary Medicine A, 1996, 43:377.

76. Fang WH Vikpur M. & Ampsandholm M. reconstructed mastitis milk by adding plasma and white blood cells to low cell count milk. Veterinary research (France), 1996, 27:33.

77. Fang WH Antagonistic effects of lactic acid bacteria on Staphylococcus aureus and Escherichia coli in Shi Ming, Huang Lin& agar plates and milk. Veterinary research (France), 1996, 27:3.

78. Fang WH Escherichia coli causing mastitis: serum sensitivity and sensitivity to selected antibacterial drugs in milk. Journal of Dairy Science, 1996, 79:76.

79. Fang WH Analysis of the efficacy of antibacterial drugs in milk. Szlig- fluorometric method based on glucuronidase. Journal of Veterinary Pharmacology and Medicine. Therapeutics, 1995, 1:422.

80. Fang WH Vikpur M. & Sandholm M. A fluorometer. Szlig- glucuronidase test is used to analyze the bacterial growth in milk. Veterinary microbiology, 1995, 46:36 1.

8 1. Marbe, klassen, Fang WH. , Myllys V, Vikerpuur M, Nyholm K, Sankari S, suoranta K. & comparison of selenite and selenium yeast feed additives on selenium binding, mastitis and leukocyte function in selenium-deficient dairy cows. Journal of Veterinary Medicine A,1995,42:11.

82. Fang WH. Inhibition of protease activity in breast milk. Journal of Dairy Research, 1995, 64:6 1.

83. Fang WH Shi Ming, Chen Jing and Huang Lin & Comparison of bacterial growth in milk and whey. Veterinary Medicine of China, 1994, 1( 1):32.

84. Fang WH Shi Ming, Chen Jing, Huang Lin&The growth of bacteria in whey was determined by turbidimetry. Veterinary Medicine of China, 1994, 1( 1):28.

85. Fang WH Shi Ming, Huang Lin & Growth of Lactic Acid Bacteria, Staphylococcus aureus and Escherichia coli in Normal and Mammary Milk and Whey. Veterinary microbiology,1993,37:115.

86. Fang WH Jiang Chun & The epidemiology and control of mastitis in several dairy herds in southeast China. Preventive Veterinary Medicine, 1993, 15(2-3): 169.

87. Hu Sheng, Fang, Lu Hairen, et al. Effects of nipple dipping and dry milk therapy on mastitis in a commercial dairy herd in China. Preventive Veterinary Medicine, 1990, 10:( 1-2), 9 1-96.