1. Do not enhance, blur, move or delete local areas of the picture.
2. Brightness, contrast or color balance can be adjusted, but it must be done on the whole picture, and the information of the original picture cannot be hidden, eliminated or distorted.
3. It is allowed to obtain images from different parts of the same gel, or from different gels, areas and exposed areas, and splice them, provided that clear dividing lines are used to indicate that they are from different original images, and this is explained in the illustration.
If the author can't provide the original data, the article will be cancelled.
-Save the original format (. czi)。
-Do not process the original document, but make a copy for processing.
-The processed image is saved in tif format, retaining the original information, while compressed formats such as jpg can only be used for general PPT. Avoid using lossy compression, tif format +lzw compression is the standard operation.
-Image adjustment should be applied to the whole picture, avoiding threshold operation and changing signal range. , and avoid using maintenance tools.
-Key image acquisition settings and processing operations need to be explained in methods or supplementary information.
Specific points are enhanced (intentionally enhancing the presentation of results) and background points are removed.
Synthetic images, by adjusting the contrast, we found that cells in other places were artificially added.
Instead of manually removing the defects on the sample, you can crop the picture, or cut off the unnecessary tissue area and take a new picture.
Technical problems unrelated to actual samples can be adjusted.
You cannot delete the background on a large scale.
Don't overdo it.
If you need to compare brightness, make sure that the shooting conditions and picture processing are exactly the same.
Don't intentionally blur or delete background information.
It is not allowed to locally decorate the strip or use contrast adjustment to mask the background.
If gaps or lines are needed for splicing, try not to splice strips from different sources.