The most important thing for uniform sampling determination is to fix some conditions!
If all the above are done, look at the RSD of your index, which should be within 5%!
In addition, when doing repeated tests, it is best to have a large sample! This can avoid some random errors! Hello, everyone. Thank you for your help. When I went to see a doctor, the dosage of medicinal materials was 500g, which was repeated three times. The results show that the relative standard deviation is about 7%. I think the rsd of these three experiments have good repeatability. Will the repetition rate be so good when extracting? In addition, when I visited, I didn't investigate the moisture absorption rate, but the soaking time was uniform. I am writing my graduation thesis now, and I don't know how to write this part of the discussion. Please help me more. In addition, is it necessary to make repeated inspections? Thank you for your repeated investigation. Of course, you have to do this.
In addition, it is related to the amount of water added. I think the extraction is simple and troublesome, but the extraction of traditional Chinese medicine itself is very difficult, but the repetition rate must be done, and it is really meaningful to transfer scientific research results to industrialization, so the repetition rate must be done, but the repetition rate really can't reach 5%. Ah, so depressed, life ah, I would like to ask which brother and sister are from the Chinese Academy of Traditional Chinese Medicine. Why isn't there a doctor's enrollment brochure there? Please answer me. I think it may be that the content of each batch of medicinal materials is different. When the same quality medicinal materials are extracted, the paste yield will vary greatly, and the parameters such as crushing and soaking will affect RSD. I suggest you try the same batch of herbs first and see how it works! Hello, everyone, I am doing the extraction of effective components from traditional Chinese medicine. After water extraction and alcohol precipitation, I passed through macroporous resin, and the repeatability of each step was not very good. Every time, the paste yield of the product and the content of effective components of the product are very poor. I want to know what are the requirements for repeatability in the process of extracting traditional Chinese medicine? Is it measured by RSD? What range should it be? Thank you all
Hello, everyone. Thank you for your help. When I went to see a doctor, the dosage of medicinal materials was 500g, which was repeated three times. The results show that the relative standard deviation is about 7%. I think the rsd of these three experiments have good repeatability. Will the repetition rate be so good when extracting? In addition, when I visited, I didn't investigate the moisture absorption rate, but the soaking time was uniform. I am writing my graduation thesis now, and I don't know how to write this part of the discussion. Please help me more. In addition, is it necessary to make repeated inspections? Thank you. There are many reasons for the poor repeatability of Chinese herbal medicine extraction process inspection. The specific analysis is as follows: 1. The uniformity of the extracted medicinal materials, although the same batch of medicinal materials, is quite different if the sampling is uneven. 2, the accuracy of sampling, sampling should be mixed sampling, content determination or paste rate determination, if a mixed sampling, the other does not do the same operation, it will cause great differences. 3. The operation influence in the process of content determination sample treatment. All these will have a certain impact on the accuracy of the results. Please carefully review whether your operation is thoughtless.
In addition, the rsd of Chinese herbal medicine extraction process is about 7%, which is quite good. If mass production, repeatability will be worse. Therefore, if the extraction rate is high, experts will not extract difficult problems in their graduation thesis.
Hehe, my personal opinion is for reference only. study . . . . . . The process of alcohol precipitation is related to Baume degree and alcohol concentration of concentrated solution, which should be strictly controlled.
In addition, the solubility of active ingredients in water and alcohol was also investigated. I extracted extracts from Ephedra, Platycodon grandiflorum, Stemona and Glycyrrhiza respectively. Is there any good extraction method? I asked my younger brother to study the extraction process by himself. The composition is extremely unstable and the repeatability is the same. However, after standardized operation, many experiments still let the truth appear. Insist on and seriously summarize, hehe, the extraction parameters of traditional Chinese medicine (dosage, times, time, solvent amount, particle size, etc. ), alcohol precipitation, macroporous resin, every step including every parameter will affect the final result, which is a complex process. Every detail will change the result of your experiment. Repeated investigation is generally necessary, and it is also a scientific verification of one's own experiments.