2. Using blast furnace slag and adding additives, a new type of high-efficiency calcium silicon fertilizer was successfully developed. "Research on the Development of New Silicon-Calcium Fertilizer and Its Efficient Application Technology" has passed the ministerial appraisal in 2006, reaching the international advanced level in general, and reaching the international leading level in improving plant disease resistance mechanism and processing technology of silicon-calcium fertilizer.
3. It is reported for the first time that the absorption and transportation of silicon by cucumber is an active process with inverse concentration gradient, which is significantly affected by low temperature and metabolic inhibitors. There are two mechanisms for higher plants to absorb and transport silicon at the same time, namely, active and passive processes, and their relative importance depends on plant species and external silicon supply concentration. This hypothesis has been confirmed in rice, corn, sunflower and wax gourd. Related papers have been published in New Botanist. It is confirmed for the first time that 68Ge can be used as a tracer to study the absorption and transport mechanism of silicon in plants. The rejection and absorption of silicon by tomatoes is an active process regulated by transporters. Related papers have been published in Plant Physiology.
4. The mechanism of improving plant resistance to abiotic stress by silicon was systematically studied and expounded for the first time. Silicon improves the activity of antioxidant enzyme system in plants, thus reducing membrane lipid peroxidation damage caused by abiotic stresses such as salt injury, freezing injury and heavy metals (cadmium, zinc, manganese, etc.). ), which is the * * * mechanism of silicon improving plant resistance to abiotic stress. The research in this field is pioneering and innovative. Related papers were published in SCI journals such as Plants and Soil, Journal of Plant Physiology, Environment and Experimental Botany, Journal of Hazardous Materials and China Science.
5. The research on the mechanism of improving the resistance of cucumber and wheat to powdery mildew, rice blast and bacterial blight by silicon has reached the international advanced and domestic leading position. Obviously, silicon itself is not an elicitor, but under the condition of bacterial infection, applying silicon to soil and other substrates can significantly improve the course-related proteins (PRs) in plants, such as POD, PPO, PAL, chitinase, chitinase and so on. -1, 3 glucanase activity enhanced the induced resistance of plants; However, foliar spraying silicon can not improve the induced resistance of plants, and it can only be achieved through "mechanical or physical barrier" or "osmotic stress" of silicate ions. Related papers have been published in Plant Pathology and European Journal of Plant Pathology. The research has passed the ministerial appraisal, and the research on improving plant disease resistance mechanism by silicon has reached the international leading level.
6. Through the research of the national support plan project "Research and demonstration of comprehensive management technology model of farmland with declining soil fertility in black soil region of Northeast China", the potential of long-term application of chemical fertilizer to inhibit soil nitrification in black brown soil region of China was clarified, while the application of organic fertilizer significantly improved the soil nitrification potential. Fertilization significantly reduced the abundance of ammonium-oxidizing archaea (AOA), but significantly increased the abundance of ammonium-oxidizing bacteria (AOB). Fertilization had no effect on the community structure of ammonium-oxidizing archaea (AOA), but significantly changed the community structure of ammonium-oxidizing bacteria (AOB). Soil nitrification potential has nothing to do with the community structure of ammonium-oxidizing archaea (AOA), but it is significantly related to the community structure of ammonium-oxidizing bacteria (AOB). Long-term application of organic fertilizer can alleviate the inhibition of chemical fertilizer on ammonium oxidation by changing the community structure of ammonium oxidizing bacteria (AOB). Related papers have been published in Microbial Ecology and Plants and Soil.
7. Through the research of 973 project "Key ecological processes and regulation affecting soil sustainable productivity", the microbial regulation mechanism of nitrogen cycle and soil ecological processes closely related to sustainable productivity are expounded; It provides a direction and way to strengthen the metabolic process of soil CNP, improve the diversity of nematodes and improve the sustainable productivity of soil, and provides a new idea for advocating and developing classical management models (such as balanced fertilization and organic fertilizer application). The research framework of the relationship between underground ecological process and soil productivity in agro-ecosystem was initially constructed. Related papers have been published in Ecological Complexity and Plants and Soil.
8. The response mechanism of rice to cadmium stress was clarified. Under stress, the activity of antioxidant enzymes and the concentration of antioxidants in rice roots and shoots decreased, which led to hydrogen peroxide "explosion", membrane lipid peroxidation and growth inhibition. Root-splitting experiments showed that cadmium could be transferred from cadmium-treated roots to non-cadmium-treated roots, and programmed cell death accelerated under cadmium stress. Salicylic acid can reduce the accumulation of H2O2 and membrane lipid peroxidation by increasing the activity of antioxidant enzymes and the concentration of antioxidants in rice, especially by binding to the sulfhydryl group of cadmium, thus reducing cadmium-induced peroxidation damage and improving the resistance to cadmium toxicity. Related papers have been published in SCI journals Environmental Pollution, Journal of Plant Physiology and Journal of Plant Nutrition.