2. 1. 1. Mechanical damage
Soak three repeated 100 seeds in 1% sodium hypochlorite solution 10 minute.
The seeds with damaged seed coat expanded obviously, and were counted by hand mirror. Split (%)
Obtained by passing 200 grams of seeds through a 4.00 mm round hole sieve. The material
Passing through a sieve is called "splitting" and then weighing (anonymous, 1985).
2. 1 evaluation of bean seed quality
2. 1. 1 Mechanical damage
Soak 100 bean seeds in 1% bleaching solution 10 minute. The obvious swelling damage of seeds and testa can be observed through hand-held lens. The bursting index was obtained by sieving 200g seeds with a 4 mm round hole sieve. Raw materials passing through the screen are called "broken" and then weighed.
2. 1.2. Germination test
Germination ability is measured according to the official seed analysts association.
(anonymous, 198 1). Three replicates of 100 seeds were placed on pre-soaked germinated paper.
Then it was placed in a seed germinator at 25 1℃ for 7 days. After 7 days, the percentage of normally germinated seeds was recorded.
2. 1.2 Germination ability test
Germination ability is defined according to the official seed expert society. Soak 100 bean seeds with three kinds of treatments on the germination test paper in advance, and then put them on the germination tester at the temperature of 25 1C for 7 days. After 7 days, the proportion of germinated seeds was recorded.
2. 1.3. Seed vigor
Seed vigor test was conducted according to the International Seed Testing Association.
(ISTA) method (anonymous, 1985). Paper towels with 350 seeds are kept in one seed.
Germinate at 25 1C for 7 days. After 7 days, the length of germinated seedlings was measured.
Centimeter. Vitality index is calculated as vitality index? 1 NX germination? %? Miao Chang? cm？ ; Where n is the number of germinated seeds.
2. 1.3 seed vigor
Seed vigor was tested according to the steps of International Seed Inspection Association. 350 bean seeds were put on paper towels and stored in a seed germination tester at 2565±438±0C for 7 days. After 7 days, the length of the seed bud was measured in centimeters. Is the vitality index based on the vitality index? 1 NX germination? %? Miao Chang? cm？ ; Where n is the number of seeds to be distinguished.
2. 1.4. Accelerated aging
This experiment is used to predict the storage potential of seeds. It combines the stress caused by the following factors
The high temperature and humidity that seeds may be exposed to. Expected seed
Batch with higher germination level recorded in accelerated aging test can be stored for a period of time.
The cycle is longer than others. 50 seeds were repeated twice and kept in perforated plastic boxes.
These boxes were kept at 40 1C and 100% relative humidity (r :h:) for 96 hours.
These batches are recorded by ISTA (anonymous, 1985).
2. 1.4 accelerated aging
This experiment is used to predict the storage potential of seeds. Including the effects of high temperature and high humidity when seeds are placed in the open air. It is expected that seeds with high records in germination ability test can be stored for a longer time than other seeds in accelerated aging test. Fifty seeds that have been treated twice are kept in perforated boxes. These boxes were placed at a temperature of 40 1C and a relative humidity of 100% for 96 hours. The germination ability of this batch is recorded by ISTA as the main point.
2.2. Determination of moisture content
The standard oven method outlined by ISTA (anonymous, 1985) was adopted. three
The replica of 10 g seed was stored in the hot air oven of 102 1C for 24 hours. At the end of this period, the sample is transferred to a dryer for cooling. The sample was then weighed and the moisture content was calculated based on the dry mass.
2.2 Definition of water content
The standard heating procedure in ISTA outline is quoted here. The 10g bean seeds processed for three times were stored in a drying box with the temperature of 102 1C for 24 hours. At the end of the test, transfer the sample to a dryer for cooling. Then weigh the sample and calculate the water content according to the drying condition.
2.3. Damage Assessment Caused by Free Fall
In order to determine the effect of falling height on seed quality, three repetitions 100 seeds,
Extract from each treatment, and fall on the cement from the height of 0.5, 1.0, 1.5 or 2.0m
Floor or galvanized iron floor. The fallen seeds are tested for germination and vigor.
Before.
2.4. Statistical analysis of data
ANOVA (anonymous, 1992) was used to analyze the data collected during the experiment to
Study the function of m:c; Effects of process stage and free fall height on seed quality parameters.
Tukey significance test is used to check the difference of average value.
2.3 Loss Assessment Caused by Free Fall
In order to determine the influence of falling height on seed quality, 65,438+000 seeds were dropped from 0.5, 65,438+0.0, 65,438+0.5 and 2.0 meters respectively. As mentioned above, the seed germination and vigor of the fallen seeds were tested.
2.4 Statistical analysis of data
The data collected in the experiment were analyzed by variance, and the effects of water content, processing platform and free fall height on seed quality were studied. The difference of detection methods was verified by Tukey significance test.