1. Obtain effective components from the prescription/traditional Chinese medicine to be studied.
2. Predict the target gene according to the effective components.
3. Obtaining the corresponding target gene according to the phenotype.
4. The target gene corresponding to the active ingredient crosses the target gene corresponding to the phenotype.
5. Construct the target gene network of effective components of traditional Chinese medicine/prescription.
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Based on the theory of system biology, the biological system network is analyzed, and specific signal nodes are selected to design multi-target drug molecules. Network pharmacology emphasizes multi-channel regulation of signal pathway, which can improve the therapeutic effect of drugs and reduce side effects, thus improving the success rate of clinical trials of new drugs and saving the cost of drug research and development.
Among them, Chinese medicine/prescription regulates the therapeutic phenotype of a certain pathway through a certain gene, and the research focuses on the detailed research, screening the target gene and pathway by means of network pharmacology, and then verifying it by conventional molecular biology and cell biology techniques. If the research details are in place, it can impact the score of SCI journal 3- 10.