Development of live attenuated marine Vibrio anguillarum vaccine (National Ocean 863 Program)
Expression of Coenzyme Q 10 Biosynthesis Gene of Escherichia coli in Photosynthetic Bacteria (National Natural Science Foundation of China)
Construction of Genetic Engineering Bacteria for One-step Fermentation of 7-ADCA (National Natural Science Foundation Project)
Design and application of molecular beacon (a key topic of Shanghai Education Commission)
Transformation of erythromycin producing strains by genetic engineering technology (enterprise horizontal cooperation project)
Construction of genetically engineered bacteria for adenosine triphosphate synthesis (enterprise horizontal cooperation project)
Genetic improvement of threonine-producing strains (horizontal cooperation project of enterprises)
Construction of Coenzyme Q 10 Genetic Engineering Bacteria (enterprise horizontal cooperation project)
Representative works since 1999
Since 2000, I have published 28 academic papers, including 24 domestic core journals, 2 in SCI and 2 in SCIE. Published 2 monographs and participated in the works 1 department. Published 4 teaching research papers.
The main work is as follows:
(0 1) Zhang Changsheng, Zhang Battle, Yao Feng, etc. Reverse transformation of lincomycin biosynthesis gene. China Journal of Antibiotics,1999,24 (2): 90 ~ 92.
(02), Wu, Zhang. The third generation genetic engineering-metabolic engineering. Pharmaceutical Biotechnology, 2000, 7 (2): 7 1 ~ 76.
(03) Jiang, Duan Shiwei and Zhang Zhanshi. Cloning and expression of Escherichia coli caiDE gene. Acta Microbiologica, 2000,49 (6): 633 ~ 637
(04) Battle of Tang Yajun, Wu, Ye Jiang and Zhang. Homologous recombination in Streptomyces Lincoln. Acta Microbiologica, 200 1, 4 1 (5): 559 ~ 566.
(05) Liang, Zeng Yang, Ye Jiang and Zhang Zhanshi. Cloning, expression and deletion of adenosine deaminase gene in Escherichia coli. Journal of Microbiology, 2002,42 (6): 686 ~ 692.
(06), Wu, Tang Yajun, Zhang Battle. Identification of homologous recombinant strains with lincomycin biosynthesis defect. China Journal of Pharmacy, 2003, 34 (4), 164 ~ 166.
(07) Lv Xiaobing, Wu Haizhen, Ye Jiang, Fan Yi, Zhang Huizhan. Expression, purification and identification of recombinant Saccharomyces cerevisiae adenosine kinase. ACTA Journal of Biochemistry and Biophysics, 2003, 35(7), 666-670.
(08) Wu Haizhen, Zhang Huizhan, Lv Caixia, Liang Na, Yi, Xing. DNA sequencing of plasmid of marine fish pathogenic Vibrio anguillarum virus ACTA Journal of Biochemistry and Biophysics, 2003, 35( 10), 956-959.
(09) Wu Haihong, Ma Yuying, Zhang Yuying, Zhang Haihong. Complete sequence of virulence plasmid pEIB 1 of marine fish pathogenic Vibrio anguillarum MVM425 and location of its replication region. Journal of Applied Microbiology, 2004,97 (5),1021-1028 (SCI)
(10) Jiang, Wu and Zhang fought. Cloning, expression and product identification of polyhydroxyalkanoate depolymerase gene from pseudomonas putida. Biotechnology express. ，2004，26(20)， 1585- 1588(SCI)
(1 1) Zhang Exhibition Editor. Introduction to genetic engineering. Shanghai: East China University of Science and Technology Press, 1999.
(12) Zhang Zhanhui editor. Pathway engineering-the third generation genetic engineering. Beijing: China Light Industry Press, 2002.
(13) edited by Ye Qin. Principles and applications of modern biotechnology (Chapter III). Beijing: China Light Industry Press, 2003.
Research direction in recent years
Microbial gene expression regulation, microbial molecular biology, genetic engineering, pathway engineering. In recent years, around the academic direction of access engineering, the main research contents focus on the exploration of the basic principles of access engineering, the development of related strategies and technologies, and the development of small molecular bioactive substances with great economic and social benefits, which are finally applied to medicine, light industry, food, textiles, environmental protection, energy and other related fields. He is especially good at studying the biosynthetic pathway of small molecular biological substances such as antibiotics, and occupies a leading position in the improvement and construction of genetically engineered bacteria for these substances in China.