Coming, coming, TA is coming again! The deep-space bianxiao, which broadcasts the latest news every day, is coming again! It's a nice day today, so it's suitable for reading the latest information and relaxing. Let's go to eat melons. The cover picture of Nature-Biotechnology 1 1 shows that the translation process of mRNA in a single cell can be quantitatively studied by using fluorescent RNA. Recently, Yang Yi and Zhu, professors of the State Key Laboratory of Bioreactor Engineering of East China University of Science and Technology, have made breakthrough progress in the field of fluorescent RNA and living cell RNA imaging after 7 years of cooperative research. Their original high-performance fluorescent RNA series has achieved fluorescent labeling and background-free imaging of different types of RNA in animal cells for the first time in the world. 165438+1October 5, this achievement was published as a cover paper in Nature-Biotechnology.

So far, no natural fluorescent RNA has been found in nature, and the few fluorescent RNA synthesized by scientists after several efforts have some problems such as low performance and difficulty in practical use. In response to this technical challenge, Yang Yi and Zhu obtained a series of fluorescent RNA with high brightness, stability and low background for the first time through brand-new molecular design and molecular isomorphism evolution.

These fluorescent rnas are compact in structure and produce strong fluorescence after specific binding with innovative dye molecules. They have different colors, such as blue, green, yellow, orange and red. Because it is similar to colorful pepper, it is named pepper. They can be inserted into different RNA molecular sequences by means of gene editing. So as to realize fluorescent labeling and real-time super-resolution imaging of these molecules in living cells without affecting their normal activities such as transcription, localization, translation and degradation. Because fluorescent RNA markers are reversible, their colors in cells can be changed at will, which is very beneficial to the construction of stable cell lines and transgenic animals with fluorescent markers.

Compared with the prior art, the fluorescence RNA is improved by 1-3 orders of magnitude in terms of affinity, stability, signal-to-noise ratio and fluorescence brightness of living cells. It embodies the breakthrough of fluorescent RNA from concept to practice and provides a valuable tool for the study of RNA function in living cells.

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