A Streptomyces strain named Streptomyces 702 was isolated and screened from soil by the Applied Microbiology Laboratory of the Department of Bioengineering, Jiangxi Agricultural University. The bioactive substances produced by Streptomyces 702 have strong antibacterial activity against cotton Fusarium wilt. By measuring its antibacterial spectrum, it was found that the bioactive substances produced by Streptomyces 702 had inhibitory effects on gram-positive bacteria and gram-negative bacteria, as well as mold and yeast. The study and determination of its stability show that it is stable to heat and ultraviolet rays and within the range of pH3-pH 12. Antimicrobial active substances were isolated and purified from the fermentation products of this strain. The results of ultraviolet spectrum, mass spectrometry, nuclear magnetic resonance and X-ray diffraction show that its active substance is the same as X2 actinomycin reported abroad, so we named it Honggumycin. Rhodotorulin is a bioactive substance produced by Streptomyces 702 fermentation, which has strong antibacterial activity. The raw material fermentation of Streptomyces 702 was studied in shake flask, and the fermentation process of raw material was explored through single factor experiment. The results showed that the seed inoculation amount was 7.5% and the antibacterial agent1.5ml. The yield of rhodopsin by shake flask fermentation reached 65438 0.46 g/L, which was 25.7% higher than that by conventional sterilization fermentation. The experimental results show that the production of rhodopsin by raw material fermentation is a potential and feasible fermentation method, which not only saves the fermentation cost, but also improves the fermentation yield.

Streptomyces 702 in the laboratory was used as the starting strain, and a rhodopsin-producing strain was screened by ultraviolet mutagenesis and NTG mutagenesis using the lethal marker mutation screening model of drug resistance gene. The stability of high-yield strains was tested by passage experiments, and the detection method of rhodopsin and the growth and metabolism model of high-yield strains were established.