The laboratory * * * published 247 papers, which were included in SCI, and many papers were accepted and published by top international magazines in this field, such as SCIENCE, PNAS and DEV. BIOL。 ，MOL。 BIOL。 EVOL. And J. VIROL Wait. All published papers are completed by aquatic science. The published papers are widely cited by international peers. According to incomplete verification, 105 SCI papers were cited 353 times by SCI source papers. At the same time, he also presided over the compilation and publication of 8 books and authorized 23 patents. Through research practice and independent innovation, a number of research achievements with great theoretical breakthroughs or broad application prospects have been formed. For example, it won the second prize of national scientific and technological progress in 2005, which is an important achievement of fishery resources regulation and efficient and high-quality model in the middle and lower reaches of the Yangtze River. Based on the principles of biological manipulation and ecological strategy, the feasible ways and main technologies of pollution-free fishery in lakes were systematically discussed, and the dynamic estimation method of the productivity of small fish and predatory fish in lakes was put forward, and the technologies of large-scale culture of mandarin fish, ecological culture of river crab and proliferation of Megalobrama amblycephala were established. "Research on Biotechnology Countermeasures for Water Quality Improvement, Reuse and Water Ecosystem Reconstruction in Tropical and Subtropical Areas" won the second prize of Hubei Science and Technology Progress in 2002. According to the ecological principle, the constructed wetland constructed by vertical flow is used for the comprehensive treatment of sewage in small and medium-sized cities, which has broad application prospects in the control of water environmental pollution; The first prize of Hubei Natural Science Award in 2003, The Genetic Basis and Breeding Significance of Two Reproductive Modes of Carassius auratus, proved for the first time with conclusive molecular genetic evidence that there are two different reproductive modes of Carassius auratus, gynogenesis and hermaphroditism, and expounded its genetic basis, and made a series of new discoveries and new understandings. The published papers are widely cited and included in internationally renowned monographs. The nutrition, feed and feeding technology of allogynogenetic crucian carp won the first prize of scientific and technological progress in Hubei Province in 2003. According to the research results of fish energetics, a high-efficient, pollution-free and pollution-free nutritional feed was developed, and a reasonable feeding model of allogynogenetic crucian carp was established, which has obvious environmental and economic benefits. In 2004, he won the second prize of Hubei Natural Science Award "Identification of Viral Pathogens of Important Aquatic Animals and Study on Pathogenic Mechanism". On the basis of establishing and screening a batch of virus proliferating cells of aquatic animals, the corresponding virus pathogens were isolated from the diseased tissues of soft-shelled turtle, frog, mandarin fish and flounder, and their pathogenic characteristics, physical and chemical characteristics, molecular structure, pathogenic mechanism and immune response were systematically studied. In 2004, the research on biological effects of space flight environment won the second prize of scientific and technological progress of weapons and equipment of the whole army of the General Armament Department. It is found that repeated reloading will still lead to new space biological effects such as character separation, and experimental evidence that plasma membrane, calcium ion and cell storage product (glycerol) * * * act on gravity perception, conduction and reaction is found, and a universal biological incubator for space biology research is designed and manufactured. China Soil Algae Research, which won the second prize of Hubei Natural Science Award in 2005, realized a large number of artificial cultivation of desert algae, invented the technology of microalgae sand fixation in arid areas, completed the experimental study of desert algae fixing wild quicksand for the first time, and opened up a new way of algae-grass-irrigation-trees comprehensive control of desertification; In 2005, he won the second prize of scientific and technological progress in Hubei Province, introduced the varieties and techniques of famous freshwater fish breeding to farmers, promoted the healthy and large-scale breeding of famous freshwater fish, promoted the creation and development of popular science works in aquaculture, and played a bridge and link role for scientific research to serve rural areas and farmers. Some of these achievements have solved the contradiction between freshwater aquaculture and water environment protection in China, some have promoted the progress and development of fish developmental genetics, aquatic virology and fishery biotechnology, and some have solved the technical problems of restoration and reconstruction of damaged urban ecosystems in China.
In addition, our laboratory is the first laboratory in the world to carry out research on genetically modified fish, and has been at the forefront of this research field. Cultivate fast-growing transgenic fish families with completely independent intellectual property rights; For the first time, the large-scale breeding experiment of genetically modified fish was systematically completed, and an efficient and safe breeding model was established. At the on-site appraisal meeting organized by the Ministry of Science and Technology, the expert group composed of three academicians unanimously recognized that the completion of this project has made China at the international leading level in the theoretical research and application prospect of genetically modified fish, and provided scientific basis for the commercialization of genetically modified fish. China Biotechnology Development Report, edited and published by the Ministry of Science and Technology, holds that the breeding of genetically modified fish in China is at the international leading level. Aiming at the ecological safety of genetically modified fish, our laboratory creatively put forward the strategy of evaluating the ecological safety of genetically modified fish in controlled isolated ecosystem by constructing artificial simulated lake, designed and constructed the world's largest artificial simulated experimental lake for ecological safety research of genetically modified fish, and comprehensively evaluated the ecological safety of genetically modified fish. In the aspect of genetic ecological security control strategy of genetically modified fish, our laboratory has achieved research results with strategic guiding significance. Transgenic fish with completely undeveloped gonads were cultivated by antisense transgenic technology. Up to now, only our laboratory can successfully control the gonadal development of fish through transgenic technology. Related research results were selected into the report of the 5th International Symposium on Fish Endocrinology, and applied for patents in China and the United States respectively. At the same time, the concept of tissue-specific bisexual complementary exogenous gene knockout was put forward for the first time in our laboratory, and the site-specific integration technology of fish transplanted genes was established for the first time, which realized the transplantation of gene knockout in fish gonads.
In the cell model study of fish antiviral genes, the differentially expressed genes induced by inactivated virus infection were cloned by subtractive technique, and a number of genes involved in fish antiviral and immune response were identified, especially interferon system genes. Since the end of 2003, more than 10 papers have been published one after another, which has attracted great attention from international peers. For example, the -PKR-like gene of crucian carp was published in Fish and Shellfish Immunology in June, 2004, and was quickly rated as one of the hot papers in this journal, which was cited by him four times, such as the paper jointly published by German and American laboratories in PNAS. We identified and characterized a gene closest to mammalian PKR from zebrafish. The expected product of this gene contains Z-DNA binding domain at the N- terminal, but does not contain dsRNA binding domain. Recently, similar genes have been reported in crucian carp. These findings support that Z-DNA binding domain plays an important role in the antiviral response of fish.
In aquatic ecotoxicology and biological monitoring, a series of technical specifications for environmental monitoring and evaluation have been formed; Established the first dedicated laboratory for dioxin research in China, and conducted sensitive and accurate detection of dioxins according to international standards; Has undertaken dozens of environmental impact assessment projects entrusted by national and local governments; Many training courses on environmental biological monitoring and water pollution control have been held at home and abroad, and a large number of technicians on environmental biological monitoring and environmental impact assessment have been trained. In the demonstration of water pollution control technology and treatment engineering, the ecological engineering of oxidation pond and sewage purification and related technologies were studied. The ecological engineering technology of pollution prevention and control and ecological optimization countermeasures in the lake area has made important achievements; The water quality management technology and sustainable utilization countermeasures based on the composite vertical flow constructed wetland system have been developed, and the research results have been popularized and applied in German, Austrian, China, Wuhan, Shanghai and Shenzhen. Focusing on the habitat improvement, ecosystem structure optimization and system stability of urban polluted water, a number of water quality improvement technologies, such as aquatic plant colonization, lakeside constructed wetland, in-situ water purification, algae control, basement restoration and hydraulic dispatching, have been developed and integrated, and a technical scheme for urban polluted water restoration combining hydraulic dispatching with aquatic plant restoration and reconstruction has been formed. The research results are not only applied to demonstration projects, but also to the planning and construction of Wuhan New District and the implementation of the six lakes connection project.
Focusing on cyanobacterial toxins that directly threaten human health and survival, we have carried out research from two aspects: basic and application. In basic research, the toxic effects of microcystins on animals, plants and other algae were systematically studied, which deepened the understanding of environmental risks of microcystins and provided scientific basis for setting the threshold of toxins in drinking water and aquatic products. The ecological effects of microcystins on aquatic animals, plants and other algae were studied. Chlorella capable of swallowing microcystins and degrading their toxins. It has the ability to distinguish toxic and non-toxic algae. This research work has important theoretical and applied value. In terms of applied research, the cyanobacterial toxin and its analytical technology system have been established and improved, and the standard microcystins and microcystin ELISA detection kits with independent intellectual property rights have occupied a place in China, breaking the embarrassing situation of the shortage of pure microcystins and imperfect detection methods in China. The microcystin ELISA kit has been widely used in environmental monitoring departments, water supply companies and scientific research institutes in China, which has created certain economic benefits, laid a foundation for the formulation of the national standard detection method of microcystin which is still lacking in China, and provided a methodological basis for the requirements of microcystin detection in June 2006 in the Water Quality Standard for Urban Water Supply promulgated by the Ministry of Construction.
Compared with the international institutions engaged in the research of algae and algae biotechnology, our laboratory is comprehensive, systematic and interconnected. Our research covers four levels: systematic classification, physiology and biochemistry, molecular genetics and environmental biology. In basic research, the ecological effects of microcystins on animals, plants and other algae are more prominent than similar studies abroad; The genetic research of cyanobacteria adaptability can be in line with the mainstream of foreign biology in terms of topics and research methods, and some new genes necessary for cyanobacteria survival adaptation or cell differentiation have been found. Systematics of COLEOPTERA is still one of the characteristics of phycology research in our institute. In application research, the research on the role of desert algae in biological crust and the development of artificial crust technology have reached the level of similar research abroad; The screening and culture technology of excellent algae species of pueraria lobata was developed, and it was the first to realize large-scale culture in China.
The study of benthic ecology aims to quantitatively clarify the structure and function of benthic community, so as to provide operational basis for the regulation of benthic system. In terms of economic benthos, the stocking model for realizing the maximum sustainable yield of river crabs was established for the first time. At the end of 2005, experts believed that this achievement was at the international leading level in the field of river crab proliferation. The study of regional limnology is devoted to exploring the macro-laws of the ecosystem in the flood plain by using comparative limnology and predictive ecology theory. A comparative limnological study of eutrophic lakes in the middle and lower reaches of the Yangtze River was carried out in order to quantitatively reveal the mechanism of eutrophication on a large scale. At the same time, the habitat fragmentation process and countermeasures of the Yangtze-lake complex system were studied in order to put forward strategic countermeasures to protect the overall biodiversity of the Yangtze River system.
With the increase of population, the demand for aquatic products is increasing, and aquaculture is also developing rapidly. At the same time, with the progress of society and the strengthening of people's environmental awareness, the development of aquatic products is increasingly facing the challenges of food safety, food safety and environmental safety. However, the research on the nutrition, feed technology and aquaculture technology of aquatic animals in aquaculture, especially intensive aquaculture, started late in China, which led to the problems of low efficiency, feed waste, environmental pollution and poor product quality in aquaculture, and these problems were all caused by the unclear response of aquatic animals to their nutritional needs, nutrient utilization and feed formula. In order to solve these problems, the nutritional requirements, protein source utilization, feeding technology and energy utilization mode of aquaculture animals were studied on the basis, which provided theoretical basis for pollution-free aquaculture. The nutritional research on fish has basically reached the same level in the world, and it is at the forefront in fish energy model and compensated growth. The research results of our laboratory are not only of theoretical significance to the fundamental needs of the healthy development of national aquatic products, but also of great significance to guiding feed formula and aquaculture. Some research results show that reasonable feed formula and improvement of feeding technology can obviously save feed, reduce nitrogen and phosphorus emissions to the environment, reduce breeding costs and reduce fishery pollution.
The demonstration of water environment engineering has had a great influence in the whole country. For example, the research project on pollution control technology of cyanobacteria bloom in Dianchi Lake has been highly concerned by national leaders. After the project passed the acceptance, Rude An Yongxiang sent a congratulatory letter; The water special demonstration project in Wuhan New District has been affirmed by the Ministry of Science and Technology and the local government. Li, Vice Minister of the Ministry of Science and Technology, said during the inspection that it is most meaningful to control water pollution only in the places where cyanobacteria bloom is the most serious. You have a team here and achieved results. The state should continue to support it, and so should you.
Important suggestions on water environment and aquatic life safety have also attracted great attention of national leaders. For example, the opening article "Genetically modified safety and countermeasures research" written for the fifth chapter of "200 1 High-tech Development Report" puts forward three countermeasures and suggestions on genetically modified safety; In 2004, he wrote a theoretical study material on transgenic and its safety for the Central Party School, expounded the origin and development of transgenic research, analyzed the essence of the debate on transgenic safety, and put forward ideas on how to correctly understand transgenic and its safety. The cultured fish species supported the local government to establish a national seed farm, which formed obvious social and economic benefits.
The international cooperation channels of the laboratory have been continuously expanded, and eight international and multilateral academic conferences have been successfully held, including the international training course on water environmental protection and water pollution control. More than 10 people work in international academic organizations, maintain long-term friendly cooperative relations with research institutions such as Germany, Japan, Britain, France, and the European Union, and organize the completion of EU projects 1. The form of international cooperation has also undergone profound changes. Researchers in our laboratory go abroad for training, degree or postdoctoral studies, and foreigners come to our laboratory for training, degree or postdoctoral studies.
In a word, all the researchers in the laboratory persist in emancipating their minds, pioneering and innovating, working hard, actively facing the national demand and the international scientific frontier, tirelessly exploring the difficult and hot issues in the frontier fields of freshwater ecology and biotechnology, striving to seek the theory and technology for protecting the healthy water environment and developing sustainable fisheries, and the research work has made great progress; At the same time, the laboratory has been completely renovated. The Institute of Hydrobiology, Chinese Academy of Sciences invested140,000 yuan, and the research platform of ecological technology and molecular biology technology was basically established. Through the continuous introduction and updating of researchers, there are 5 researchers 15 under the age of 50 in the laboratory and 5 sponsors of the National Fund for Distinguished Young Scholars. Six people were selected for the Hundred Talents Program of Chinese Academy of Sciences, and three people were selected for the National Hundred Million Talents Project. More than 40 senior visiting scholars came to the laboratory for cooperative research, and more than 20 postdoctoral students entered the laboratory through measures such as opening up projects. A team of high-quality talents with reasonable age structure, optimized knowledge structure and strong professional level has been formed. The status of laboratory in the same subject field at home and abroad and its role in national scientific and technological development and economic construction have been obviously enhanced.