Design of model drug experiment scheme;
Scutellaria barbata, 95% ethanol, berberine hydrochloride reference substance, distilled water, hydrochloric acid solution, sodium hydroxide solution and iodine-potassium iodide reagent.
Instrument:
Model 722 ultraviolet spectrophotometer, electronic analysis balance, electrothermal constant temperature water bath pot, data ultrasonic cleaning machine, miniature plant pulverizer, acidity meter, heating plate and circulating water multi-purpose vacuum pump.
Experimental method:
Ultrasonic extraction, semi-bionic method, orthogonal test, alkaloid identification method
Experimental steps:
I. Preparation of drugs and instruments
2. Preparation of reference solution, determination of wavelength, preparation of standard curve and calculation of regression equation.
Third, the preparation of sample solution
Fourthly, alkaloids were extracted from Scutellaria barbata by semi-bionic method.
1, single factor experiment
Effect of (1) temperature on extraction effect
(2) The influence of acidity on the extraction effect.
(3) The influence of alkalinity on the extraction effect.
(4) The effect of solid-liquid ratio on extraction.
2. Determination of factors and levels and orthogonal test design;
Single factor temperature, acidity, alkalinity and solid-liquid ratio are taken as the four levels of orthogonal test.
Using semi-bionic method, the optimum extraction conditions were determined by orthogonal test.
5. Ultrasonic-assisted semi-bionic extraction of Scutellaria barbata alkaloids: ultrasonic extraction was carried out under the optimal extraction conditions obtained by semi-bionic method.
1, single factor experiment
Effect of (1) temperature on extraction effect
(2) The influence of extraction times on the extraction effect.
(3) The influence of ethanol concentration on the extraction effect.
2. Determination of factors and levels and orthogonal test design;
The optimum conditions of ultrasonic-assisted semi-bionic extraction were determined by orthogonal experiments with temperature, extraction times and ethanol concentration as single factors and three levels.
6. Optimization conditions of ultrasonic-assisted semi-bionic extraction of alkaloids from Scutellaria barbata.
7. Measure the absorbance of the extracted alkaloids with an ultraviolet spectrophotometer, and compare it with the standard curve to calculate the amount and yield of alkaloids.
Eight, the identification of alkaloids:
Alkaloid precipitation reaction
1, potassium bismuth iodide reagent reaction Take 1ml percolate, add 1 ~ 2 drops of potassium bismuth iodide reagent, and if it produces brown to brownish red, it is a positive reaction.
2. For iodine-potassium iodide reagent reaction, take 1ml leachate and add 1 ~ 2 drops of iodine-potassium iodide reagent. Those who produce brownish yellow precipitate are positive.
3, silicotungstic acid reagent reaction Take 1ml percolate, add a few drops of silicotungstic acid reagent, and light yellow precipitation is a positive reaction.
Model design of experimental scheme 2 1. Experimental topic:
Study on the influence of printing conditions on the quality of precoated film
Second, the experimental design ideas:
The factors affecting the quality of laminating are mainly divided into two categories, namely, the influence of laminating process and the influence of printing conditions. The influence of laminating process is undoubtedly the temperature, speed and pressure of laminating. At present, most of the factors that affect the quality of film mulching are the factors (temperature, speed and pressure) that affect the film mulching process, such as wrinkling, foaming, curling, peeling, peeling, overlapping and other quality factors. However, the influence of printing conditions on the quality of laminating is rarely considered. But sometimes it is also because of the printing conditions that affect the quality of laminating. Based on this, the direction of this experimental study is to specify certain film covering conditions, and to make proofing and pre-coating by changing the conditions required for printing samples. The final experimental result is to discuss how printing conditions affect the quality of pre-coated film, and whether there are certain rules to follow. Finally, on this basis, the best printing conditions of film covering of printed matter are determined.
Three, experimental instruments and materials:
(1)IGT printability tester, precoated laminating machine and peeling strength tester;
(2) Ink, coated paper (determine specifications and ph value) and BOPP precoated film.
Fourth, the experimental preparation:
Testing and proofing are carried out in IGT printability instrument to determine the printing condition range of general printing proofing. (approximate range of temperature, speed and pressure).
Five, the experimental principle and steps:
(1) The coating quality was investigated by coating strength index, and the coating strength was determined according to GB2790- 1995 standard. For each sample, the average peeling force was measured from the relationship curve between peeling force and peeling length, and the maximum and minimum values of peeling force within the peeling length of at least 100mm were recorded in units of n, and the corresponding peeling strength values were calculated.
The calculation formula is as follows:
? 180 = catering department
Where:? 180 is the peel strength (n/mm); F is the peeling force (n); B is the sample width (mm). Using the above formula, calculate the average peel strength, minimum peel strength and maximum peel strength of all test samples, and their arithmetic mean.
(2) Proofing on coated paper with IGT printability tester.
1. Carry out on-site printing proofing under certain ink layer thickness, printing speed and printing pressure. After proofing, dry the spline in normal printing environment (drying time is affected by paper, temperature and humidity). Splines with different printing intervals (0.5h, 1h, 1.5h, 2h, 2.5h, 3h, 3.5h, 4h, 4.5h, 5h, 5.5h, 6h, 6.5h, 7h) were coated on the precoating press (the coating pressure was 6. 8mPa, temperature 99. Then, the peel strength record data of each strip is tested on the peel strength tester to determine the best film covering time interval.
2. Under the condition of the best printing time interval determined in the first step, the printing pressure and printing speed are unchanged, and the ink layer thickness is changed for on-site proofing, and the printed splines with different ink layer thicknesses (0.5-4.0 μm) are coated with the same film, and the peeling strength of each spline is tested on a peeling strength tester, and the data is recorded. Determine the best ink layer thickness of film covering.
3. Under the conditions of the best printing time interval and the best ink layer thickness obtained in the first two steps, control the printing pressure to be constant, and change the printing speed (0. 1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0m/s) to carry out on-site printing proofing. Determine the best printing speed.
4. According to the best printing time interval, the best ink layer thickness and the best printing speed obtained from the previous three experiments, the printing pressure (200-800N) was changed to carry out on-site printing proofing, and the printed splines were covered with the same film under different printing pressures, and the peeling strength of each spline was tested on a peeling strength tester, and the data was recorded.
Six, data processing:
Seven, the experimental results:
Eight. References:
Qi, Printing Materials and Applicability, Beijing: Printing Industry Press, 2008.6438+0 (Undergraduate Planning Textbook for Printing Engineering in General Higher Education)
Xu Wencai, Packaging, Printing and Post-press Processing: China Light Industry Press 20 10.8 (Textbook for the Tenth Five-Year Plan of National General Higher Education)
Feng Ruigan, Printing Principle and Technology Beijing: Printing Industry Press 1999-4 (Printing Engineering Textbook for Colleges and Universities)
GB- T2790- 1995, peel strength test method of adhesive 180b [S]
Model essay on experimental scheme design III. Training purpose
Graduation thesis design is a very important concentrated practice link for students to comprehensively use what they have learned to analyze and solve practical problems.
Through the implementation of this link, students are guided to master the methods of thesis writing, learn to investigate and study, complete the design and writing of graduation thesis, get exercise in the practice of thesis writing, improve their ability of analyzing and solving problems, enhance their innovative consciousness and professional quality, and become a qualified graduate who is good at investigation and research, speech and writing.
Second, the paper design steering group
Team leader: Xu Wuhai, Lu Lunwen
Members:,, Wen,, Bu Jianli.
Third, the paper design (writing) requirements
Graduation thesis is an important teaching link that students must complete before graduation. Students are required to use the basic knowledge, basic theories, skills, tools and methods they have learned in school to study and discuss related problems in practical work. It is an important means to comprehensively examine students' ability to analyze, solve and operate problems with what they have learned. In the process of thesis writing, students are required to:
1. On the basis of seeking truth from facts and going deep into reality, write a graduation thesis with certain quality independently by using the knowledge you have learned.
2. The topic selection of graduation thesis should be carried out in the form of academic papers, research reports or analysis reports within the scope of the major studied.
3. The graduation thesis should be novel, clear and innovative; The materials are informative and powerful; The structure is complete and accurate; The language is accurate and fluent.
4. The graduation thesis shall be in the unified format required by standardization (see the unified format of the Ministry), and the number of words in the text shall be 10000- 15000 words.
Fourthly, the design and implementation of the paper.
1, organize mobilization
Time: 65438+00 on April 27th.
Venue: Joule (50 1)
Object: All students of International Trade Grade 08.
Mode: collective mobilization meeting
The class teacher (counselor) should assist in organizing and mobilizing.
2. Student Registration Group
Graduation thesis is a compulsory part for students, and it is not allowed to be exempted. Graduates of 1 1 should submit their applications before May 1. Group according to the registration situation. Each tutor guides a group of students. In principle, each group should not exceed 18.
3. Instruct the appointment of teachers
The graduation design instructor is a full-time teacher with corresponding major, bachelor degree, strong practical ability and certain teaching experience. The instructor is responsible for guiding the whole process of students' graduation design.
The duties of the lecturer are:
(1) Instructors should conscientiously perform their duties and guide students to organize the whole process of graduation thesis design;
(2) Instructors should be responsible for the direction, thinking, structure, format and quality of the paper, and sign opinions at the designated position of the finalized paper as required;
The main contents of the review comments include: whether the topic is appropriate and whether the theme of the paper is clear; Whether the structure is reasonable and the expression is accurate and smooth; Whether the selected materials are appropriate, sufficient and representative; Whether the logic of the discussion is reasonable, etc.
(3) Instructors should urge students to contact teachers in time and submit the writing outline, first draft, revised draft and first draft on time.
(4) The instructor should record the guidance in the work record book;
(5) Instructors should guide each student's thesis for not less than 5 hours/week.
4. Students design (write) papers
Students determine the topic under the guidance of the instructor. Requirements for topic selection:
(1) should meet the professional training objectives and teaching requirements, should not be divorced from the professional scope, should be comprehensive, with a certain depth and breadth;
(2) The topic size is moderate, which has certain guiding significance for practical work;
(3) Thinking should be combined with current hot and difficult issues to encourage the solution of practical problems;
(4) once the topic is determined, it will generally not be changed.
In the process of thesis design (writing), the instructor should help students solve specific problems in the process of design (writing). After the paper is completed, the instructor must write the preliminary evaluation results and comments that conform to the whole paper design process.
5. Timetable (***5 weeks)
Mobilization and topic selection stage: April 27-May10;
Drafting the outline of the paper: May10-24;
First draft of design (writing) thesis: May 25th-June 24th;
Revision stage: June 25th, 20XX-June 20th, 20xx.
Thesis submission and preliminary evaluation defense stage: 20XX from the end of June to July.
Fifth, the paper design performance evaluation
The graduation thesis design score is expressed by a percentage system, which is given by the tutor according to the students' writing attitude and the quality of the thesis. Divided into excellent, good, pass and fail.
1, excellent (above 90 points)
(1) conforms to the relevant principles and policies of the party and the state;
(2) The topic of the paper is clear, it can integrate theory with practice, and it has certain uniqueness and reality in the study of economic work or academic problems, and it has some new ideas;
(3) The central argument of the paper is prominent, the argument is sufficient, the argumentation process is logical, the article structure is reasonable, the expression is smooth and the level is clear.
2, good (80-89 points)
(1) conforms to the relevant principles and policies of the party and the state, can apply what they have learned, integrate theory with practice, have clear views and make in-depth analysis;
(2) The topic of the paper is clear, which has certain practical significance and can be used to analyze real economic problems with the knowledge learned;
(3) The center of the paper is prominent, the arguments are sufficient, the argumentation process is logical, the article structure is reasonable, the level is clear, and the expression is smooth.
3, pass (60-79 points)
(1) conforms to the relevant principles and policies of the party and the state, and can basically use the knowledge learned to analyze problems, but the content is insufficient;
(2) The thesis has a clear argument and can still be linked with the actual economic work;
(3) The information in the paper is still fully specific, but the content is outdated, lacking originality, insufficient arguments and unconvincing. The article is organized and the words are fluent.
4. Fail (below 60 points)
Anyone who meets one of the following conditions will fail.
(1) does not conform to the relevant principles and policies of the party and the state, or has a principled error in economic theory, or has not mastered relevant professional knowledge and lacks written skills;
(2) improper topic selection, lack of central idea and main line of discussion, chaotic structure, unclear level, lack of logic, lack of main arguments, disjointed arguments or serious improper collocation;