1, through the breeding of excellent strains, the research of fermentation process control and the proposal of new online separation technology, it has a significant role in promoting the industrial large-scale production of lactic acid, and also has certain reference significance for the fermentation production of related microorganisms. In the breeding research of heat-resistant lactic acid bacteria, the combination of physical and chemical mutagenesis and chemostat greatly improved the screening efficiency of forward mutant strains, reduced the workload of strain screening, and also had a certain reference for strains with tolerance in other screening environments. The suitable temperature for the growth of lactic acid bacteria is about 37℃. When cultivating high-temperature resistant lactic acid bacteria, it is necessary to raise the temperature of the growth environment, and most lactic acid bacteria will die in this process. Therefore, it is necessary to observe the growth of lactic acid bacteria in time, and when its growth state changes, take it out of the high temperature environment for further confirmation and research. However, lactic acid bacteria are anaerobic, and frequent removal of low-temperature resistant lactic acid bacteria will have an impact on other lactic acid growth environments.

2. The history of human application of lactic acid bacteria is very long. In ancient times, people unconsciously used lactic acid bacteria in brewing and food. However, it has been nearly a hundred years since human beings actively studied and mastered the life law of lactic acid bacteria and applied it. In recent years, with the improvement of people's living standards and the needs of national economic development, the brewing industries such as dairy products, soy sauce, liquor and beverages have begun to study and utilize lactic acid bacteria and achieved gratifying results. In order to make the research and application of lactic acid bacteria in the wine-making industry more in-depth, this paper features it in order to play a role in attracting jade.