Virus-free and in vitro propagation are the most widely used and effective aspects in plant tissue culture. Many asexual crops are infected by virus in production, which leads to serious degradation of varieties and reduction of yield and quality. Using plant meristem for culture can remove viruses from new plants. Crops producing virus-free seedlings by this method include potato, sweet potato, garlic, strawberry, apple, banana and so on. , and has been applied to production on a large scale. In vitro rapid propagation technology can make the amplification speed of some plants tens of thousands or even millions times faster than that of conventional methods, and the generated seedlings have unified genetic background and good repeatability, and are not limited by seasons and regions. At present, many tissue culture factories with an annual output of millions of seedlings have been built in the world, which has become a new industry. In vitro rapid propagation technology has been widely used in ornamental plants, horticultural plants, economic trees and asexual propagation crops.