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Study on HeLa cells in the establishment of mouse cervical cancer model
HeLa cells are often used to construct mouse cervical cancer models, and are widely used in cell culture, biological experiments and tumor research. The appearance of HeLa cells points out the direction for the treatment of cervical cancer. When discussing drugs and methods for the treatment of cervical cancer, we often study the effects of compounds on the invasion and migration of HeLa cells, and on the survival of cervical cancer tumor inhibition model mice.

——HeLa cell, a cell line widely used in human biology, comes from 195 1, a cervical cancer cell from an American woman, and has now become a very important tool in medical research. Human cells are difficult to survive for a long time because of the limited number of divisions, and tumor HeLa cells have tenacious vitality and fertility, so they became the first human cell line obtained by scientists. HeLa cell line is transformed from normal cervical cancer cells into cancer cells by human papillomavirus, which is different from normal cervical cancer cells in many aspects. The cell was positive for keratin immunoprecipitation staining, containing the sequence of human papillomavirus-18, with normal expression level of pRB and low expression level of p53. Human papillomavirus (HPV) plays an important role in the pathogenesis of cervical cancer. HPV 16 and HPV 18 are two important subtypes of human papillomavirus.

Since 1952, HeLa cells have contributed more than 60,000 papers, including the research and development of polio vaccine and the research that led to two Nobel Prizes. There are also numerous studies on the construction of mouse cervical cancer model using HeLa cells. If some researchers find that the mouse cervical cancer model established by self-labeling human cervical cancer cell Hela with green fluorescent eggs can provide ideal experimental materials for human cervical cancer research, then the living animal imaging system should be used to objectively and quantitatively evaluate the growth of living tumor cells in animals, rather than the change of tumor volume. In the preliminary screening of pharmacodynamics or evaluation of curative effect of anticancer drugs, mouse tumor transplantation model is often used for in vivo experiments. Medici can provide various effective tumor transplantation models to test the effectiveness of drugs according to the needs of customers. Conventional tumor diseases include: breast cancer, lung cancer, colon cancer, kidney cancer, DLBCL lymphoma and so on. Animal experiments were carried out with rats, mice and hamsters.

The mouse cervical cancer model constructed with Hela cells can also explore the mechanism of the compound on colon cancer. For example, some researchers have discussed the radiosensitization and protective mechanism of black garlic extract on HeLa cell transplanted tumor of cervical cancer. By establishing a subcutaneous transplanted tumor model of HeLa cells in mice, mice were randomly divided into four groups: blank control group, γ -ray irradiation group, black garlic group and black garlic+γ -ray irradiation group. Normal saline, γ -ray irradiation, black garlic extract and black garlic extract combined with γ -ray irradiation were used respectively, and then the growth rate of transplanted tumor in each group was observed [1]. It was found that the extract of black garlic had radiosensitization effect on HeLa cell transplanted tumor. By enhancing immune function, the peripheral blood white blood cell count and the body's antioxidant ability are improved, thus improving the body's radiation protection ability.

Some researchers have also discussed the effects of zedoary on the proliferation and immune function of human cervical cancer cells [2]. The researchers randomly divided 50 nude mice transplanted tumor models into 5 groups and gave them different treatments to observe the changes of their immune function and the pathological results of cancer tissues. TT method was used to detect the inhibitory effect of zedoary turmeric oil decoction on cervical cancer Hela cells The results showed that Rhizoma Curcumae could inhibit the growth of cervical cancer cells and promote the apoptosis of cancer cells.

Cervical cancer is one of the most common malignant tumors in gynecology. The mouse cervical cancer model was established by the tumorigenesis experiment of Hela cells in nude mice. By observing the effect of the compound on transplanted tumor in nude mice, it can lay a foundation for the development of anti-tumor drugs. In a word, Hela cell line is an important tool for past and future medical research.

[1] Study on radiosensitization and protective mechanism of black garlic extract on HeLa cell transplanted tumor of cervical cancer [J].

[2] Effect of Rhizoma Curcumae on proliferation and immune function of human cervical cancer Hela cells [J].