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How to write the materials and methods in the paper?
First, the description of materials should be clear and accurate. Generally, the materials are summarized first, and then the structure, main components or important characteristics of the materials and the functions of the equipment are described in detail. The material description shall clearly indicate the number, source and preparation method of the research object. If an instrument with a trademark name is used, it should also include an accurate technical description of the instrument, and some even require the name and location of the instrument and sample manufacturer.

The experimental materials should use common names familiar to international counterparts, and try to avoid using special names known only to the author's domestic counterparts. If products with different characteristics are known and there are important differences between them, such as specific microbial culture medium, it is necessary to use the trademark and the name of the manufacturer to express the difference from the common name, and the trademark name will be added after the common description.

Animals, plants and microorganisms used in experiments should be accurately identified, and their sources, special properties, sampling requirements or standards should be explained. When it is necessary to describe the types of various microorganisms or the sources and characteristics of compounds, a list can be used; Otherwise, briefly describe it in the text, footnotes or illustrations. If the research object is a person, we should pay special attention to the specific requirements for contributing journals, explain the selection criteria of the research object, and consider the general important statistical characteristics and other statistical information related to the theme of the article according to the situation.

Second, the description of the method should be detailed and appropriate, highlighting the method, that is, describing how the research is carried out. Methods are usually described according to the time sequence of research steps, which generally includes the experimental environment or conditions, the method of selecting the research object, the reasons for choosing specific materials, equipment or methods, the experimental procedures, the applied statistical analysis methods, etc. If there is no chronological order, describe the experimental steps in order of importance.

In the description of the method, enough detailed information should be given so that colleagues can repeat the experiment and avoid mixing the contents about the results or findings. If necessary, the reasons for choosing a specific method should be fully explained. If the method is novel and has not been published, all necessary details should be provided. If the method used has been publicly reported, it is enough to quote relevant literature. If the journal reporting this method has limited influence, you can describe it in a little detail.

A detailed description of the statistical analysis method of data usually shows that the author has recently designed or obtained this method, and the author thinks that readers need this explanation. Generally speaking, ordinary statistical methods do not need comments or explanations, while advanced or unusual statistical methods need to be properly cited.

If there are many things to describe, you can use subheadings in different levels and try to create subheadings corresponding to the contents in the conclusion. This kind of writing can keep the consistent echo within the article, and readers can quickly understand a specific method and its related results. In addition, before submitting the manuscript, the author can ask colleagues to read the final draft to find out whether the description of the method is clear or obviously omitted.