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How to analyze the problems of biopharmaceutical process flow analysis
Biotechnology pharmacy includes genetic engineering pharmacy, cell engineering pharmacy, enzyme engineering pharmacy and fermentation engineering pharmacy. The technological process of genetic engineering pharmacy can be divided into the following steps: 1. The target gene can be obtained by various methods, such as reverse transcription, shotgun shooting, chemical synthesis or transformation from existing genes. 2. When combining the target gene with the vector to construct engineering bacteria, it should be noted that the vector must meet the following requirements: it has multiple cloning sites, strong promoters and terminators, and can independently replicate iodine forging, fermentation and culture, that is, it is cultured in a fermentor. 4. Expression of exogenous target gene. 5. Purification and separation of exogenous expression products generally include the following steps: cell crushing, solid-liquid separation, concentration, preliminary purification and high purification. Cell engineering pharmacy can be divided into animal cell pharmacy and plant cell pharmacy. At present, animal cell pharmacy mainly produces monoclonal antibodies and vaccines. Plant cell pharmacy is mainly to obtain the metabolites of plant cells in this life. This method has the advantages of easily controlled conditions and high yield. The disadvantage is that heredity is not stable enough. Enzyme engineering pharmacy combines enzyme engineering with protoplast fusion, induction and gene recombination, which not only doubles the efficiency of microbial transformation, but also makes the whole production process continuous and automatic. This method of fermentation engineering pharmacy is also called microbial engineering. On the basis of the original fermentation process, new technology was adopted, which greatly improved the technological level. It can be regarded as a part of genetic engineering pharmacy. Both methods obtain a large number of target products through large-scale cell culture. This method needs to find the strain first, and then expand the culture.