Zhanqin Zhao, male, born in 1980, doctor, professor, doctoral supervisor, academic leader of the veterinary biological products engineering team of Henan University of Science and Technology. Graduated from Huazhong Agricultural University in 2008, majoring in preventive veterinary medicine. In the same year, he went to the College of Animal Science and Technology of Henan University of Science and Technology to engage in teaching and scientific research. From 20 15.05 to 20 16.05, he was a visiting scholar at the College of Veterinary Medicine, University of Georgia, USA. Long-term commitment to scientific research, teaching and production, commitment to scientific research, personnel training and technical services. The research direction is the research and development of livestock infectious diseases and their vaccines. Basic research focuses on people and animals suffering from bacterial infectious diseases, mainly engaged in the cross-species transmission mechanism of "super bacteria" such as multidrug-resistant Escherichia coli and Mycobacterium tuberculosis from animals and the correlation between their host susceptibility. In the applied research, we focus on vaccines for important bacterial infectious diseases of livestock and poultry, and study the biology, technology, development and creation of veterinary biological products in depth.

Chinese name: Zhanqin Zhao.

Mbth: Zhan qinzhao

Nationality: China.

Ethnic group: Han nationality

Place of birth: Lankao, Kaifeng, Henan

Date of birth: 1980. 10

Occupation: teacher

Graduate school: Ph.D., Huazhong Agricultural University.

Faith: China party member.

Main achievements: animal infectious diseases and vaccine research and development.

Representative works: research and development of vaccines for major bacterial infectious diseases in pigs

outline

Zhanqin Zhao, male, born in 1980, doctor, professor, doctoral supervisor, academic leader of the veterinary biological products engineering team of Henan University of Science and Technology. Graduated from Veterinary Engineering College of Henan Agricultural University in 2002, majoring in veterinary (health inspection); In the same year, he went to the State Key Laboratory of Agricultural Microbiology of Huazhong Agricultural University to study for a doctor's degree (master's degree, doctor's degree) in preventive veterinary medicine. The tutors are Academician Chen Huanchun and Professor Wu Bin, famous experts of livestock infectious diseases. During my doctoral studies, I was mainly engaged in the isolation and identification of Streptococcus suis, Escherichia coli, Bordetella bronchiseptica and Haemophilus parasuis, as well as the serotype and genotyping research. During this period, he won the "Excellent Postgraduate of the State Key Laboratory of Agricultural Microbiology" award. The doctoral thesis "Study on Recombinant Salmonella Vaccine of Bordetella bronchiseptica" won the Excellent Doctoral Thesis Award in Hubei Province.

Since 2008, he has been engaged in teaching and scientific research in the College of Animal Science and Technology of Henan University of Science and Technology, of which 20 15.05-20 16.05 was a visiting scholar in the College of Veterinary Medicine of the University of Georgia. Long-term commitment to scientific research, teaching and production, commitment to scientific research, personnel training and technical services. The research direction is the research and development of livestock infectious diseases and their vaccines. Basic research focuses on people and animals suffering from bacterial infectious diseases, mainly engaged in the cross-species transmission mechanism of "super bacteria" such as multidrug-resistant Escherichia coli and Mycobacterium tuberculosis from animals and the correlation between their host susceptibility. In the applied research, we focus on vaccines for important bacterial infectious diseases of livestock and poultry, and study the biology, technology, development and creation of veterinary biological products in depth. A complete germ plasm resource bank of livestock and poultry pathogens was established, including more than 5,600 strains of major animal bacterial pathogens such as Haemophilus parasuis, Streptococcus, Bordetella bronchiseptica, Pasteurella multocida and Erythrina erysipelas, and 32 vaccine strains were screened out in line with the epidemic characteristics in China. The protective antigens of more than 70 important pathogenic bacteria were identified by modern molecular biology technology, and the combined vaccine and subunit vaccine of 5 bacterial infectious diseases were developed and reformed. He presided over the National Natural Science Foundation, major horizontal projects of school-enterprise cooperation and other scientific research projects 16, won the second prize of Henan Science and Technology Progress 18, and published 52 research papers in domestic and foreign journals such as Infection and Immunity, Vaccine, China Agricultural Sciences and Journal of Microbiology as the first author or correspondent. Among them, there are 2 SCI papers1piece, 2 national new veterinary drug registration certificates, 6 books edited or edited, and national invention patents 1 1 piece.

Main research experience

1.2003.07-2005.06 Master's degree, mainly engaged in the isolation and identification of Escherichia coli in swine edema disease, inactivated vaccine and diagnostic methods. Completed the research stage of the vaccine laboratory, including the screening of Escherichia coli vaccine strains for edema disease, the biological characteristics of the strains, the preparation of inactivated vaccine, the efficacy test of mouse virus attack protection and the efficacy test of pig virus attack protection. Guide the completion of the late clinical trial of inactivated vaccine.

2. From June 2005 to April 2006, he participated in the diagnosis and tackling key problems of swine streptococcosis in Sichuan in 2005: the main executive appraiser of swine streptococcosis in Sichuan in 2005. The molecular biological characteristics of Sichuan virulent strains SC- 1, SC-2, SC-3 and other local virulent strains were studied. It laid a foundation for the development of swine streptococcus vaccine. At the same time, a clinical practical PCR rapid detection kit for Streptococcus suis type 2 was developed.

3. From May 2006 to August 2007, the epidemiology of Bordetella bronchiseptica, the main pathogen of atrophic rhinitis in pigs, and the development of genetic engineering vaccine. Including the isolation, identification and molecular biology of Bordetella bronchiseptica, the development of salmonella vector, and the development of Bordetella recombinant salmonella genetic engineering vaccine. The constructed genetic engineering recombination vaccines has passed the environmental release test. At the same time, it obtained 1 national invention patents.

4. Since 2002, pig farms all over the country have carried out a lot of veterinary clinical diagnosis and comprehensive prevention and control work. Especially in large-scale pig farms in Hubei, Henan and other provinces for long-term clinical diagnosis and disease epidemic investigation. A large number of first-hand epidemiological data of pigs were obtained, and a large number of epidemic strains were isolated, including streptococcus, Haemophilus parasuis, Pasteurella multocida and Bordetella bronchiseptica.

5. Since 2008, he has been engaged in teaching and scientific research in the College of Animal Science and Technology of Henan University of Science and Technology. His research direction is the research and development of livestock infectious diseases and their vaccines, focusing on vaccines for important bacterial infectious diseases of livestock, and conducting in-depth research on the biology, technology, development and creation of veterinary biological products. A complete germplasm resource bank of pathogenic bacteria of livestock and poultry was established, and more than 5,600 strains of main pathogenic bacteria of animals were preserved, and 32 vaccine strains which meet the epidemic characteristics of China were screened out. The protective antigens of more than 70 important pathogenic bacteria were identified by modern molecular biology technology, and the combined vaccine and subunit vaccine of 5 bacterial infectious diseases were developed and reformed.

Research foundation and technology platform

The main research direction is the research and development of livestock infectious diseases and their vaccines. Basic research focuses on people and animals suffering from bacterial infectious diseases, mainly engaged in the cross-species transmission mechanism of "super bacteria" such as multidrug-resistant Escherichia coli and Mycobacterium tuberculosis from animals and the correlation between their host susceptibility. In the applied research, we focus on vaccines for important bacterial infectious diseases of livestock and poultry, and study the biology, technology, development and creation of veterinary biological products in depth. At present, a large number of research materials and technical means have been accumulated, and the following main research foundations and technical platforms have been laid.

A complete germplasm resource database of livestock and poultry pathogens was established, including more than 5,600 strains of major animal pathogens such as Haemophilus parasuis, Streptococcus, Bordetella bronchiseptica, Pasteurella and erysipelas. 32 vaccine strains were screened out, which were in line with the epidemic characteristics in China. The protective antigens of more than 70 important pathogenic bacteria were identified by modern molecular biology technology, and the combined vaccine and subunit vaccine of 5 bacterial infectious diseases were developed and reformed. He presided over the National Natural Science Foundation, major horizontal projects of school-enterprise cooperation and other scientific research projects 16, won the second prize of Henan Science and Technology Progress 18, and published 52 research papers in domestic and foreign journals such as Infection and Immunity, Vaccine, China Agricultural Sciences and Journal of Microbiology as the first author or correspondent. Among them, there are 2 SCI papers1piece, 2 national new veterinary drug registration certificates, 6 books edited or edited, and national invention patents 1 1 piece.

"Open Laboratory of Key Discipline of Environment and Animal Products Safety in Colleges and Universities in Henan Province" is an excellent laboratory for "double-base" evaluation in Henan Province. The school has animal science and technology college, affiliated animal hospital, medical college and affiliated "National Top 100 Hospitals", which is suitable for carrying out scientific research projects such as animal infectious diseases and zoonosis. ABIVeriti PCR instrument, BIO-RADGelDoc gel imaging system, BIO-radprotean 12 two-dimensional electrophoresis system, Alpha 1-2LD vacuum freeze dryer, Sigma3- 18KS high-speed freeze centrifuge, multifunctional bacterial cell transformation instrument, ultrasonic cell disruption instrument, PKF bacterial endotoxin detector, BIO-RAD protein two-dimensional electrophoresis instrument, Thermo ultra-low temperature refrigerator, ultra-pure water system, ultraviolet spectrophotometer, automatic enzyme label instrument, Antai SW-CJ-2FD.

The laboratory open platforms of College of Animal Science and Technology of Henan University of Science and Technology include BIO-RADCFX96Touch fluorescence quantitative PCR instrument, Axioboserva1inverted fluorescence microscope of Zeiss company, FACSVerse flow cytometer of BD company, optimum-80XP ultra-high speed freezing centrifuge of Beckman company, AKTAPure protein purification system of ge company, AllianceE2695 high performance liquid chromatograph of WATERS company, Clarus690 gas chromatograph of PerkinElmer company, PinAAcle900H atomic absorption spectrometer of PCR company, Avio200 inductively coupled plasma emission spectrometer of PerkinElmer company, Nanodrop2000C ultramicro nucleic acid protein concentration analyzer of PerkinElmer company and Thermo company, etc. There are also experimental animal rooms, animal hospitals, experimental pastures, sterile cell rooms and other facilities.

The open platform of dynamic laboratory of Henan University of Science and Technology has large-scale instruments and equipment such as HitachiS4800 electron scanning microscope and HitachiHT7700 transmission electron microscope.

Major scientific research projects undertaken

1. national natural science foundation project, isolation and identification of NDM- 1 bacteria from pigs and study on the possibility of human-pig interaction (3 1302 106), 20 14-20 16250000.

2. National Natural Science Foundation Project, Study on Transmission Characteristics of Metal β-Lactamase-1 Escherichia coli from New Delhi (U17041Kloc-0/7), 20 18-2020520000 yuan.

3. Major horizontal project of school-enterprise cooperation, research and development of trivalent inactivated vaccine of Haemophilus parasuis (H20 160922), 2016-20195.2 million yuan.

4. Major horizontal project of school-enterprise cooperation, research and development of joint inactivated vaccine of Streptococcus suis and Haemophilus parasuis (H20 170806), 2017-202.244 million yuan.

5. A major horizontal project of school-enterprise cooperation, research and development of pentavalent inactivated vaccine of Haemophilus parasuis (H20 190 107), 2018-202.248 million yuan.

6. Horizontal project of school-enterprise cooperation, development of video system for diagnosis and treatment of veterinary infectious diseases (H20 18 1205-2), 20 19.438+0-2020438+0232 million yuan.

7. The major Industry-University-Research Cultivation Fund Project of Henan University of Science and Technology (2015ZDC04) 2016-201950000 yuan.

8. Young Academic Leaders Fund Project of Henan University of Science and Technology, Study on Subunit Vaccine of Sheep Infectious Pleuropneumonia (2015xd28), 20 15-20 17300000 yuan.

Major research papers published