Real-time fluorescence quantitative PCR detection system is also called real-time fluorescence quantitative PCR detection system. The core of QCPR is the real-time fluorescence quantitative qPCR instrument and its supporting detection and analysis software system. The principle of polymerase chain reaction (PCR) won the 1993 Nobel Prize in chemistry for its sensitivity, specificity and rapidity. Because of its unique advantages in pathogen detection, developed countries quickly developed related methods and instruments, which became the mainstream of molecular biological diagnosis. It is still at the forefront of academic and application. Up to now, there are three generations of products: the first generation of PCR qualitative detection technology and equipment consists of DNA thermal cycler+electrophoresis instrument+uv analyzer+qualitative reagent; The second generation PCR-DNA endpoint quantitative PCR detection consists of gene amplification thermal cycler+fluorometer+endpoint quantitative reagent, which can be divided into endpoint ELISA-PCR and endpoint fluorescence -PCR. The third generation product is real-time QCPR-DNA/RNA real-time fluorescence quantitative detection.

QPCR has the outstanding advantages of high detection sensitivity, wide detection linear range, good detection accuracy and repeatability, so it is recognized as the most advanced nucleic acid molecular diagnosis technology in the world for clinical and scientific research, and is recognized and respected by the FDA of the United States. Detection items include: food and other pathogenic bacteria and disease viruses and other pathogens such as HBV DNA, HCV RNA of hepatitis C virus, HIV H ⅳ RNA, Chlamydia Trachomatis CT, Neisseria gonorrhoeae NG, cytomegalovirus CMV, Mycobacterium tuberculosis Mtb, avian influenza, Enterobacter sakazakii, foot-and-mouth disease, Newcastle disease, classical swine fever, Escherichia coli O157: H7, Salmonella and Bacillus anthracis.

The application of PCR is very extensive, involving almost all life science fields: food inspection, clinical inspection, disease control, inspection and quarantine, scientific research laboratory, food safety, cosmetics inspection, environmental sanitation and so on. Many domestic reagent manufacturers have produced dozens of fluorescent quantitative PCR kits, including hepatitis, sexually transmitted diseases, prenatal and postnatal care, respiratory diseases, avian influenza, Enterobacter sakazakii, foot-and-mouth disease, Newcastle disease, classical swine fever, Escherichia coli O 157∶H7, Salmonella, Bacillus anthracis, Actinobacillus pleuropneumoniae, parasitic diseases and so on. , easy to buy, cheap, and get the relevant national certification.

It is worth mentioning that in view of the frequent epidemic diseases and the practical application of real-time fluorescence quantitative PCR technology, real-time fluorescence quantitative PCR detection technology has been forcibly applied to related industries at home and abroad, and international, national and industry standards have been formulated as legal basis. For example:

SN/T 1632.3-2005 detection method of Enterobacter sakazakii in milk powder fluorescence PCR method

GB/T 19438. 1-2004 universal fluorescent RT-PCR detection method for avian influenza virus.

Gb/t19438.2-2004 fluorescent RT-PCR detection method for H5 subtype avian influenza virus

Compared with expensive imported instruments, it is a necessary choice for domestic users to choose high-quality domestic fluorescent quantitative PCR instruments whose quality and performance are comparable to those of imported instruments, and the price is only one third or even one fifth of that of imported instruments, and it is also advocated by the national health authorities, the Clinical Laboratory Center of the Ministry of Health and the State Food and Drug Administration of the United States. The use of domestic fluorescent quantitative PCR instruments with quality and performance comparable to imported instruments can save a lot of foreign exchange for the country, enable more users to have advanced real-time fluorescent quantitative PCR technology and equipment, and at the same time promote the technological progress of domestic instruments, the development of national industries, and benefit the country and the people.

The TL988 real-time fluorescence quantitative PCR instrument produced by Xi An Tianlong Technology Co., Ltd. has been tested by the testing center designated by the State Administration of Pharmaceutical Products, and the National Quality Inspection and Quarantine Bureau has recognized CNAL global mutual recognition testing qualification, and its performance has reached the international advanced level of similar products. Through clinical trials in two national drug clinical bases, we obtained the medical device registration certificate issued by SFDA of the US Food and Drug Administration, which conforms to the management norms and work norms of clinical gene amplification laboratories issued by the Ministry of Health.

Since 2005, Chinese researchers have carried out research on the classification, pathogenic mechanism, hazard assessment, pollution reduction measures, detection methods, instruments and standards of Enterobacter sakazakii. Related research papers include:

Biological characteristics and health hazards of Enterobacter sakazakii, Pei Xiaoyan, Liu Xiumei, Institute of Nutrition and Food Safety, China Center for Disease Control and Prevention.

Enterobacter sakazakii in dairy products can cause infant death, China Food Industry Network May 28, 2008 10:56.

Development of real-time fluorescence quantitative PCR instrument in dairy detection, Peng Niancai, Su, Zhang Zhenxi, China dairy industry, CN23-1177/TS200705: 62-64;

Rapid detection of Enterobacter sakazakii in dairy products, Peng Niancai, Li Ming, food safety guide, CN11-5478/R2008.07;

Enterobacter sakazakii in infant milk powder and its detection, Peng Niancai, Zhang Zhenxi, Su, China Dairy, CN 1 1-4768/S, August 2008.

Rapid detection and analysis of highly harmful food-borne pathogen Enterobacter sakazakii in dairy products, Peng Niancai, Inner Mongolia dairy industry P 18-20, March 2008.