It is against this background that Sachs pioneered the method of hydroponics to study and measure the metabolism of plants. In this way, Sachs learned that plants can fully grow and develop in nutrient solution without soil. Because, experiments have proved that plants survive by absorbing nutrients dissolved in water in the soil. This confirms the understanding that "carbon dioxide can form organic matter in plants" put forward by Ingenhousz and others.

This hydroponic experiment of Saks strongly proves that the carbon-containing compounds in plants come from carbon dioxide in the air, not from soil organic matter. At the same time, saxophone's work also attracted the attention of Staerck Halter, a scholar of Talantailin College. So he hired saxophone as a physiological assistant in the college, and saxophone worked here for three years.

After working in Tarantula College for three years, with the help of his research achievements in plant nutrition, Sachs found another job as a teacher in Bobersdorf Agricultural College near Bonn. From 186 1 to 1865, he completed the Handbook of Plant Experimental Physiology, which he started writing when he was in Tarantula College, and taught at Bobersdorf Agricultural College. This book was later published in Leipzig in 1865, making saxophone the founder of experimental plant physiology.

From 65438 to 0866, Sachs was hired as a professor at the University of Freiburg in Braez. Two years later, Sachs was hired as a professor at the University of Wü rzburg. At the University of Wü rzburg, Sachs launched his research work on plant physiology in an all-round way. His research fields involve plant embryology, plant nutrition, the effects of light and dark conditions on plants, the growth form of plants and how water moves in plants. He wants to draw a blueprint for the new discipline of plant physiology, point out the direction for future research in this field, and make more adequate preparations for future research in methods and equipment.