2. Study on bioactive substances based on traditional Chinese medicine. This paper mainly studies the bioactive substances in anti-stroke, hypolipidemic and anti-tumor Chinese medicines.
3. Microbial pharmacy. It mainly studies drug biosynthesis and biotransformation, bioactive substances produced by microorganisms and so on.
The research technology involves the separation and purification of bioactive substances, structural analysis, microbial culture and fermentation, molecular biology, genetic engineering, enzyme engineering, animal cell culture, animal experiments, organic synthesis and so on.
Scientific research projects:
Responsible for completing the research work of 7 scientific research projects such as National Natural Science Foundation, Shandong Natural Science Foundation, Ministry of Health and Ministry of Education Fund. As the project leader, he is undertaking the research work of six projects, including "Study on the Treatment of Neurodegenerative Diseases with Stichopus Japonicus Glycopeptides".
Representative academic papers:
1. Tang Jinbao, Li, Ji,, Zhu Peng. Optimization of secretory expression conditions of ProZZ-EGFP fusion protein gene in Escherichia coli. China Journal of Biochemical Pharmacy, 2007, 28 (1), 37-39.
2. Tang Jinbao, Song Ji, Zhu Peng. Fusion expression and activity determination of staphylococcal protein A "ZZ" and EGFP. China Journal of Microbiology and Immunology. 2007，27 (6)，532-535。
3. Wang Li, Ji. Prediction and analysis of protein group secreted by Escherichia coli UTI89. Biotechnology in China. 2007,27 (7), 100- 105.
4. Jiang Sufen, Ji, Song, Wang Weili. Research progress of hippocampus in China. Chinese herbal medicine. 2007, 30(7), 884-887.
5. Song, Tang Jinbao, Ji, etc. Extraction, purification and content determination of Gynostemma pentaphyllum polysaccharide. Chinese herbal medicine. 2006, 29(6), 595-597.
6. Ji, Wang Weili, Chen. Research progress of bioactive substances from Stichopus japonicus [J]. China Journal of Biopharmaceutical. 2006,27 (5): 52-54.
7. Chen, Ji, Wang Weili,. Recent development of marine antimicrobial peptides exhibition. China Medical Information.2006,4 (7): 30-32.
8. Zhu Peng, Ji,. Attenuated Salmonella as a carrier of human endostatin gene is used in tumor gene therapy. China Journal of Biochemical Pharmacy, 2006,27 (2): 38-40.
9. Song, Ji. Central nervous system function of phosphatidylserine. China Journal of Biochemical Pharmacy .2006,27 (2):13-15.
10. Fangfang, Ji. Preparation of 3- benzyloxy -4- methoxyphenylacetic acid. China Journal of Pharmacy.2006.37 (2): 80-81.
1 1. biotransformation in organic chemistry, Ji, translated, June 2006, Beijing: Chemical Industry Press.
12. Translated by pharmaceutical biotechnology Ji, June 2005, Beijing: Chemical Industry Press.
13. Li Congfa, Ji, Tian and Qu. Determination of epoxide in cell suspension by dual-wavelength microplate method. Journal of analytical science. 2004，20 (3): 245-247。
14. Participated in microbial enzyme transformation technology. June 2004, Beijing: Chemical Industry Press.
15., Ji, Li Congfa. Separation of chiral drug intermediates by high performance capillary electrophoresis with sulfonylated β -cyclodextrin chiral selector. Chinese journal of modern applied pharmacy magazine. 2004,2 1 (3): 188- 190.
16. Li Congfa,,, Ding Di, Ji & Yin Boqu. Resolution of ethyl 3- phenylglycinate catalyzed by epoxide hydrolase. Biotechnology Bulletin, 20031February; 25(24):2 1 13-2 1 16.
17. Li Congfa, Tian, Ji and Qu. Screening of epoxide hydrolase-producing bacteria and study on enzyme-producing conditions. Journal of Microbiology, 2003,43 (3): 422-426.
18., Liu, Ji. Determination of phenyl ethylene oxide by ultraviolet-visible microplate spectrophotometry. Physical and chemical inspection, 2003,39 (9): 554-555.
19. Li Congfa,,, Ding Di,, Yin. Catalytic resolution of ethyl 3- phenylglycinate by epoxide hydrolase. China chemical alphabet. 2003, 14( 1 1): 1 133- 1 134.
20. Cui Hongjie. Ji aiguo Li congfa Qu yinbo. Xie Yunying. Resolution of benzyl glycidyl ether, a chiral drug intermediate, on HPLC stationary phase, Chemical World 2003,44 (9): 466-467,490.
2 1. Ji aiguo, Michael miller, Michael Wahlberg, Werner hummel and Christian wadley. Synthesis of optically pure tert-butyl (4S, 5R) 4- methyl -5- hydroxy -3- carbonyl hexanoate by chemical enzymatic method. Organic chemistry. 2002,22 ( 12): 66.
22. Meng, Ji and Li Congfa, Qu. Synthesis of key intermediates of ubenimex. Chinese journal of medicinal chemistry.2002.6 (3):168-169.
23. Ji, Pei Ji, Gao. Substrate selectivity of 2,5-diketo -D- gluconic acid and 2- keto -L- gulonic acid produced by Gluconobacter oxydans in multienzyme system. Applied biochemistry and biotechnology. 200 1,94(3):2 13-223.
24. Michael Wahlberg, Ai Guo Gee, Werner hummel and Michael Miller. Enzymatic reduction of hydrophobic β, δ-diketone ester. Synthesis 200 1, 6:937-942.
25. Ai Guo Gee, Michael Wahlberg, Werner hummel, Siesti Ann Vandre and michael moorer. Dynamic kinetic resolution of tert-butyl 4- methyl-3,5-dioxanohexanoate by enzymatic reduction. Chemical newsletter. 200 1.57-58.
26. Ji, Gao Peiji. Preparation of CoI-Sepharose 6MB affinity gel and its application in purification of 2,5-diketone -D- gluconate reductase. Journal of Shandong Medical University. 2000，38 (3): 258-259。
27. Ji, Gao Peiji. Study on substrate transformation characteristics of Gluconobacter oxydans and immobilization of Corynebacterium cells to synthesize 2- keto -L- gulonic acid. Pharmaceutical Biotechnology.1999,6 (1):14-19.
28. Ji, Gao Peiji. Synthesis of 2- keto -L- gulonic acid from gluconic acid by co-immobilization of Gluconobacter oxydans and Corynebacterium. Biotechnology express. Vol. 20,No. 10, 10 1998, pp. 939-942.
29. Ji Aiguo. Study on synthesis of iron chelating agent 1- hydroxy -2- ethyl -3- hydroxypyridine -4- one. China Journal of Pharmacy.1998,29 (5): 203-20.
Teaching courses:
Postgraduate courses include pharmaceutical biotechnology, chemical biology, research and development of marine drugs, marine microorganisms and their metabolites, chiral and chiral drugs, and undergraduate courses include marine biopharmaceuticals, introduction to biomedicine and pharmaceutical enzyme engineering.
Representative achievements and themes:
As the project leader, he completed 2 scientific research projects of the National Natural Science Foundation. As the project leader, he has undertaken or completed many other types of scientific research projects, such as the Ministry of Education, the Ministry of Health and the Shandong Provincial Research Fund. Published more than 50 academic papers at home and abroad.