Zhu Weifeng Luo Daya Tu Lixia Jie Kemin Wan et al.

Objective to establish a rapid oral swab with salting-out method

Extraction method of plant genomic DNA. Methods Oral epithelial cells were digested with cell lysate and protease K, and then 5 mol/L was used.

Protein was precipitated with NaCl, and DNA was precipitated with isopropanol. Finally, the obtained DNA was washed with 70% ethanol and dissolved in Te (10 mmol/L Tris-HCl, 1 mmol/L).

Etta, pH

8.0). PCR-RFLP technique was used to analyze the rs 1042522 and CHRNA3 genes of TP53 gene.

Rs 129 10984 locus was used for typing. Sequencing verification of different genotype samples. Results The amount of DNA extracted from a single oral swab was between 0.68 and 2.56 μ g, and the ratio of D260/D280 was 0.65.

The ratio is between 1.77 and 1.94. After PCR amplification and digestion, two single nucleotide polymorphisms of 10 samples were clearly typed. The restriction enzyme digestion results were consistent with the sequencing results. Conclusion The results of this experiment are as follows

The salting-out method can quickly, simply and economically obtain high-quality genomic DNA from oral swab.

Author:

Department of Biochemistry and Molecular Biology, medical college of nanchang university;