Shanghai second military medical university press under the guidance of biochemical experiments

Author: editor Zhang

ISBN: 10 bit[78 10607685] 13 bit[97878 1060768 1]

Press: Shanghai Second Military Medical University Press

Release date: August 2007-1

Pricing: 25.50 yuan This book is based on the new biochemistry textbook and aims at cultivating practical talents. Based on the new teaching plan and syllabus, we mainly focus on cultivating students' basic experimental skills so that students can understand and master the basic experimental methods of biochemistry. The book is divided into three chapters, including biochemical experiment technology, 23 biochemical experiments and student experiment reports.

This book is concise, novel and practical. It can be used as a teaching book for medical college students and a desk reference book for medical and health workers. The first biochemical experimental technology

Chapter 1 The purpose and laboratory rules of biochemical experiments

Chapter II Basic Skills Training

Chapter iii chemical reagents

Chapter IV Formulation of Preparation Reagents

Chapter V Preparation of Experimental Samples

Chapter VI Commonly Used Biochemical Experimental Methods

Chapter VII Experimental Error and Data Processing

The second biochemical experiment

Experiment 1: protein amphoteric dissociation and isoelectric point determination.

Experiment 2 protein denaturation and precipitation

Experiment 3 serum cellulose acetate membrane electrophoresis

Experiment 4: The total serum protein was determined by biuret method.

Experiment 5 Determination of serum albumin (bromocresol green method)

Experiment 6 enzyme specificity and factors affecting enzyme reaction

Column chromatography of experimental heptaterpenes

Experiment 8 Determination of blood sugar

Experiment 9 Qualitative determination of urine sugar (Bancroft reagent method)

Experiment/competitive inhibition of 10/0 succinate dehydrogenase

Experiment 1 1 acetylacetone chromogenic method for determination of serum triacylglycerol.

Experiment/determination of serum total cholesterol in kloc-0/2

Experiment 13: formation of ketone bodies in liver

Experiment/qualitative determination of ketone bodies in kloc-0/4 urine (Langer method)

Experiment 15 transamination

Experiment/determination of serum alanine aminotransferase (ALT) activity in kloc-0/6 (modified Reichsler method)

Experiment/determination of serum urea in kloc-0/7 (diacetyl oxime method)

Experiment/Extraction and Component Identification of Kloc-0/8 Yeast RNA

Experiment 19 DNA extraction and quantification from animal liver

Experiment 20 Determination of serum total bilirubin and bound bilirubin (improved J-G method)

Experiment 2 1 urine protein qualitative experiment (sulfosalicylic acid method)

Experiment 22 Determination of serum potassium and sodium (flame photometry)

Experiment 23 Determination of Plasma Carbon Dioxide Binding Capacity (Titration)

Appendix 1 Common normal biochemical values

Appendix II Conversion Table of Commonly Used Biochemical Units

Appendix III Relationship between Relative Density and Concentration of Acid and Alkali Commonly Used in Laboratory

Appendix IV Atomic Scale of Elements

Appendix V Preparation Methods of Common Buffers

Appendix VI Common Acid-base Indicators

refer to

Experimental report of biochemistry students