In the fourth episode of the third season of Rick and Morty, all the superheroes who belong to this cartoon appeared, which is the first time that superheroes appeared. They are in the guardian alliance, they are the first line of defense when the evil forces arrive, and they are the guardians of the uninformed people, which is very similar to Guardians of the Galaxy's first description.

The first action of the Guardian Alliance took place in the summer of Rick and Morty's first or second season. Rick thinks he is Morty's hero. Rick was unfriendly in the first action when he found that Morty admired the members of the Guardian Alliance more. So in the second operation, the Guardian Alliance didn't call Rick.