From infected corn and soybean seeds or seedlings, or directly from

Soil has a series of pathogenicity and aggressiveness.

Pathogen detection. So, the agents of these pathogens and organisms

In order to understand the effect of seeds on germinated soil, some experiments were carried out.

Petri dish test

Pathogen and metastasis of mold and fungus accounted for 65438 0% respectively.

(w/v) water agar (wa) and incubation room temperature.

(25E30 _ c) Inoculated 7 days ago. Soybean seed

The surface was sterilized and placed in fungal colony (WA). seed

It was also placed on the unvaccinated Wa plate as a control. plate

Hatched at 10 _C in the dark. Will you come and move the room in six days?

25e30 _C) temperature (2 days before the disease.

I'm evaluating the disease. x 0e4？ Remember that the copy (me) and n are total?

Check the number of seedlings.

Preparation of inoculum in fungal soil

All fungal colonies potato glucose agar should be planted in and

The incubation time at 25e30 _C is 3 days. Six 1 cm 2 plugs 3 days ago

A culture of growing potato glucose agar was placed in each fungus.

A 500ml flask contains 200ml of sand,11.2ml of corn and.

Deionized 80 ml water (autoclaved twice, then lasted for 40 minutes

In another 24 hours). Fungi can be parasitic on corn.

/kloc-eating on 0/0. This is a shaking bottle.