Targeted modification of animal genes

The combination of gene editing and propagation techniques (such as bovine embryo culture in vitro) allows genome editing directly in fertilized oocytes using synthetic highly specific endonucleases. CRISPR -Cas9 further increases the application scope of gene editing in targeted modification of animal genes. CRISPR-Cas9 allows direct injection of cytoplasm to achieve one-time simultaneous knockout of multiple targets of mammalian fertilized eggs. .

Single cell gene expression analysis solved the transcription roadmap of human development and found the key candidate genes for functional research. Using genome-wide transcriptome data to guide experiments, genome editing tools based on CRISPR make it possible to interfere with or delete key genes to clarify their functions.

Targeted modification of plant genes

Directed modification of plant genes is the most widely used field in gene editing. First, we can modify endogenous genes to help design the required plant traits. For example, important trait genes can be added to specific sites of main crops through gene editing, and their * * * separation in the breeding process can be ensured through physical connection, which is called "trait accumulation".

Secondly, it can produce herbicide-resistant crops. For example, using ZFN-assisted gene targeting, two herbicide-resistant genes were introduced into crops. Thirdly, it can be used to prevent and control many diseases such as banana stripe virus.

In addition, gene editing technology has also been applied to improve the quality of agricultural products, such as improving the quality of soybean oil and increasing the storage potential of potatoes.