What is the best absorbance at maximum absorption? If the concentration is too large, the absorbance of the absorption peak exceeds 4, is that good? Used for graduation thesis.
Atlas is certainly not good. Your absorbance reaches 4, indicating that the sample is opaque and the measured data is wrong. According to Bill-Lambert Law: A=-logT. When A=0, the transmittance is 100%. When A=3, the transmittance t is basically equal to 0, that is, no light passes through. There must be something wrong with the sample you configured. Generally, the recommended absorbance range is 0.2-0.7. Sometimes 1.5 can be measured. You measured 4, don't take it out, please handle the sample.