It plays an active and evil role in many nationalities in the world. Being bitten by a snake and its venom remind people of the fear of death, but the agility and mystery of the snake deeply attract the symbol of alchemist. Snakes shed their shells again and again, arousing people's reverie and the mystery of rejuvenation and eternal youth. Humans are both afraid of snakes and full of fascination and awe. This kind of love-hate relationship with snakes has existed in many ethnic groups at all times and in all countries. This is a common cross-cultural phenomenon. In Christian tradition, snakes often become synonymous with evil, which reminds people of Satan or the devil. For example, the snake in Genesis 3 is interpreted by many people as the incarnation of Satan or the devil. However, our negative view of snakes is just one of many images of snakes. In the Bible, snakes present various faces, which deserve our in-depth understanding. This paper aims to explore the significance of different snake images and symbols in ancient Near East and Israel. The theme of the first chapter of this paper discusses the symbolic significance of snakes in ancient near east culture, and discusses the different roles and functions played by snakes. This paper mainly uses the method of ancient iconology to show the image and symbolic significance of snakes. Several representative patterns are selected to illustrate: Kudulus in Mesopotamia, Amdoua in ancient Egypt, and clay sacrificial vessels unearthed in Beitan, Palestine. The second chapter of this paper discusses the theme of snakes in the Bible. Analyze several passages in the Old Testament and present different meanings of snake. Because the theme of the scripture also involves the myths, religions and worldviews of the ancient Near East, it can be seen that the theme of snakes is closely related to the cultures and religions of all ethnic groups in the ancient Near East. In the past, the Christian churches in Taiwan Province Province held a negative understanding of the snakes in the Bible, which had a negative impact on the dialogue between the gospel and culture. Finally, this thesis has a modest expectation, trying to provide discussion materials for the local theological work in Taiwan Province Province by studying the theme of snakes. I hope to open up a space for dialogue and theological reflection between the material of local culture and the theme of Bible text.