Title: Professor, Doctoral Supervisor
SETTING: Department of Polymer, School of Materials, Tianjin University.
Research interests: polymer gel, non-viral transgenic vectors and drug delivery vectors, soft tissue engineering, functional polymer materials 199 1.7, and obtained a bachelor's degree in polymer chemical engineering from the Department of Applied Chemistry of Tianjin University.
1996.3 obtained the master's degree in engineering from the Department of Applied Chemistry of Tianjin University.
1999.4 obtained the doctor's degree in biomedical engineering from the School of Materials, Tianjin University.
1999.7 Teacher, Polymer Department, School of Materials, Tianjin University.
2003.7-2004.438+0 Visiting Scholar, Department of Orthopaedics, School of Medicine, University of Hong Kong
2004.3-2004.6438+0 1 Research Assistant, Hong Kong Polytechnic University
From March 2005 to June 2006, 65438+February, she was a postdoctoral researcher at the School of Medicine, University of Ottawa, Canada, mainly engaged in the research of tissue engineering and non-viral transgenic vectors, and constructed a corneal substitute based on human recombinant collagen for the first time, which is expected to solve the problem of scarce corneal donors. Remarkable achievements have been made in chitosan-based carriers, nonionic transgenic carriers, stimulus-sensitive carriers and quantum dot carriers with good biocompatibility. The condensation effect of chitosan carrier on DNA was systematically studied, and the conformational changes of DNA and the topological structure changes of the complex were investigated. It is pointed out that the strong electrostatic interaction between chitosan and DNA is an important factor to limit gene expression, and thus a hydrophobically modified efficient chitosan derivative transgenic vector is constructed. A temperature-sensitive transgenic vector that can regulate gene packaging and unpacking was created, and a molecular recognition transgenic vector based on hydrogen bonding with DNA base pairs was innovatively constructed. The essence of DNA condensation is systematically studied, and the design idea of non-viral vector is expanded. A non-viral vector with high efficiency and low toxicity which can be degraded by intracellular redox was constructed, and the influence of polymer branching structure on the complex structure and intracellular buffer was revealed. A non-viral transgenic vector with low toxicity and in-situ imaging based on ZnO quantum dots and nano-diamond was designed, which expanded the application scope of ZnO quantum dots and nano-diamond. In recent years, more than 90 papers have been published in internationally renowned material journals such as advanced materials, biomaterials, advanced functional materials, biomacromolecules, J controlled release, biological conjugate chemistry, J Mater Chemistry, etc., and others have cited and commented 1800 times. He applied for 20 domestic invention patents and granted 15 patents, and won the first prize of Tianjin Natural Science (ranked second).
In 2006, he was selected into the New Century Excellent Talents Program of the Ministry of Education, and was supported by the National Science Fund for Outstanding Young Scholars on 20 13. Member of Chinese Chemical Society, American Chemical Society and British Chemical Industry Association.
Deputy Director of Youth Working Committee of Tianjin Biomedical Engineering Society;
Main papers published in recent years
Yang Jianhai, Li, Mei Griffith, Liu *, cationic polymer brush grafted nano-diamond was used to enhance gene delivery and biological imaging through atom transfer radical polymerization. J Material Chemistry 20 1 1, 2 1, 7755-7764.
, Han Yanjiao,,, Liu *. Construction of ultra-high strength hydrogel with excellent fatigue resistance based on strong dipole-dipole interaction. Soft substance 20 1 1, 7:2825-283 1.
, Yang Yang, Liu *. The powerful MeO(2)MA/ vinyl-4,6-diamino-1 3,5-triazine copolymer hydrogel mediated reverse gene transfection and heat-induced cell separation. Biomaterials 20 1 1, 32: 1943- 1949.
Dai and Liu *. PDM aema- polythiobetaine diblock copolymer enhances gene transfection and serum stability of the complex. Biomaterials 20 1 1, 32:628-638. IF7.37
, Wen Guang, Liu *, Liu Guipei. High-strength hydrogel with integrated functions of hydrogen bonding and temperature-responsive surface-mediated reverse transfection and cell separation. Advanced materials 20 10, 22:2652-2656.
Dai, Liu Yongjie and Liu *. Redox cleavable star cation PDMAEMA is used as a non-viral vector for gene delivery through the arm-first method of ATRP. Biomaterials 20 10/0,31:559-569.
Viral vectors of,,, and ZnO @ co-PDM aeman for plasmid DNA delivery and biological imaging. Biomaterials 20 10/0,31:3087–3094.
Yang Jianhai,,, Sun Peng, Liu *, Sun Pei, Zuo,. Temperature-regulated DNA concentration and gene transfection of non-viral vectors based on PEI-g-(PMEO(2)MA-b-PHEMA) copolymer. Biomaterials 20 10/0,31:144-155.
McLaughlin CR, Acosta MC, Luna C, Liu WG, Belmonte C, Griffith M, Gallar J. Regeneration of functional nerves in guinea pig full-thickness collagen-choline phosphate corneal replacement implant. Biomaterials 201031(10): 2770-2778.
Merrett K，Liu WG，Mitra D，Camm KD，McLaughlin CR，Liu Y，MA，Li F，Griffith M，Fogg DE。 A new synthetic sugar polymer-recombinant human collagen hybrid is used as a bionic crosslinking agent in corneal tissue engineering. Biomaterials 2009,30 (29): 5403-5408.
Liu, Christopher mclaughlin, Pell Fagerholm, Neil S. Lagall, Belinda Haine, Juan C. Schiano, Mitchell A. Watsky, Jean Munger, Shozaki Naoki, Li Fengfu, May Griffith. Collagen-phosphatidylcholine interpenetrating network hydrogel as corneal substitute. Biomaterials 2009,30 (8):1551-1559.
, Liu, Lin Lin, Chen Dayong, martine H. Degradable disulfide cross-linked micelles prepared by vinyl functionalized nucleoside through R AFT process are used as drug delivery systems. Biomacromolecules 2008, 9:332 1-333 1
Dai, Wang, Tang, Lei, Yang Jianhai, Liu, *, Li, He Xian,. Double-temperature-responsive poly (betaine-based ABA) triblock copolymer with DNA condensation ability. Polymer 2008, 49: 5322-5328.
Liu, Kimberly Mei Lite, May Griffith, Per Fagerholm, Subhadra Dravida, Belinda Heyne, Juan C. Scano, Mitchell A. Watsky, Shinozaki Zhishi, Neil Lagall, Rejean Munger and Li Fengfu. Recombinant human collagen for tissue engineering corneal substitutes. Biomaterials 2008,29:1147-1158.
, Liu, *, Liang Dongchun, Guo Gang,. A novel non-viral vector based on poly (vinyldiaminotriazine) was designed by specific hydrogen bonds with nucleic acid base pairs, advanced functional materials 2007, 17:246-252.
, Liu *, Chen Dayong,, Lu. Effect of block sequence of ABA and BAB NIPAAm/ HEMA triblock copolymers on thermal response behavior of solution. Polymer Chemical Physics 2007,208:1773–1781.
, Liu, *, Ji,,, Guo Gang,. Study on temperature-sensitive non-viral transgenic vector of poly (N- isopropylacrylamide)/poly-arginine. Biomaterials 2006,27: 4984-4992.
Sun Shujun,, Liu,,, Zhang,, Yao,,, Zuo, Guo Gang,. A temperature-sensitive chitosan -nipa am/ vinyl laurate copolymer carrier for gene transfection. Bioconjugate Chemistry 2005, 16 (4): 972-980.
, Liu *, Sun Shujun,,, Yao, Lu. Study on physical and chemical properties of chitosan /DNA polyelectrolyte complex, biomaterials 2005, 26:2705-27 1 1.
Sun Shujun, Liu, et al. A temperature-responsive chitosan -nipa am/ vinyl laurate copolymer carrier for gene transfection. Bioconjugate Chemistry 2005, 16 (4): 972-980.
Liu, et al. Synthesis and characterization of temperature-sensitive N- isopropylacrylamide -N- propylacrylamide-vinyl pyrrolidone terpolymer and its preliminary application as embolic agent, Biomaterials 2005,26 (34): 7002-70/1.
, Liu, *, Yao,,,,. Understanding of thermal response transition behavior of hydrophobic modified N- isopropylacrylamide copolymer solution, Polymer 2005,46 (14): 5268-5277.
Liu *, Zhang, Lu, Zhu Dunwan, Yao, Zhao Chengru, Wang Chuandong. A sol-gel reversible poly (N- isopropylacrylamide) -g- methylcellulose copolymer hydrogel with rapid temperature response, biomaterial 2004, 25:3005-30 12.
, Liu, Sun Shujun,,, Yao,, Guo Gang,. N- alkylated chitosan as a potential non-viral vector for gene transfection, Biobinding Chemistry 2003, 14(4): 782-789.
Liu, Yao, chitosan and its derivatives-a promising non-viral gene transfection vector, controlled release journal 2002, 83:1-1.
Participate in book chapters
At the invitation of Professor Kevin J. Yarema of Johns Hopkins University in the United States, chitosan-based non-viral gene delivery vector, in Handbook of Carbohydrate Engineering, US Eastern Time. Kevin J. Arema (Johns Hopkins University), Taylor &; Francis Group, May 27, 2005.
Meg Griffith, Perfagholm, Liu et al. Corneal regenerative medicine: corneal substance for transplantation. Corneal and external eye diseases. Edited by T. Lienhard and F. Larkin. Pages 37-53. In 2007, Heidelberg, Berlin, springer. Awards:
1. "Chitosan-based bionic polymer gel" won the first prize of Tianjin Natural Science, 2002ZR- 1-004.
2. In 2002, he was awarded the title of Excellent Teacher of Tianjin University.
3. First Prize of Academic Thesis Tutor at Tiannan University Academic Festival in 2006
Teaching courses:
Undergraduate course: soft matter and its medical application (innovative course of the whole school)
Postgraduate course: biomaterials