Methods: The concentration of phage was purified to 10 12/ml by mpeg precipitation and chloroform extraction. The effects of concentration of spinning solution, high flow rate and high voltage on the diameter and morphology of spinning fiber were observed by scanning electron microscope after the film was prepared by adjusting PVA concentration, high flow rate and spinning. A molecularly imprinted membrane with PVA as the "shell" and Escherichia coli phage as the "core" was prepared by electrospinning technology, and the encapsulation of bioactive phage bitten by agar was observed by double etching method.
Results: The phage successfully concentrated reached 10 12/ml. In the preparation process, the membrane 2 with PVA concentration of 7%(w/w) was found by using water as solvent. Before adding ethanol and SM, the spinning was unstable, and a large number of broken filaments and beads appeared. The liquid membrane could not be continued because of the time, and the experiment was terminated. Relationship between open morphology, PVA spinning fiber diameter, PVA concentration and voltage
Conclusion: (1) The observation results of several films show that with the increase of spinning solution concentration, the fiber diameter increases gradually, the fiber diameter distribution increases, and the voltage does not change obviously with the increase of fiber diameter.
2. The experiment of polymer matrix phage and the spinning compatibility of PVA were evaluated.
[Keywords:]: phage maximization electrospinning PVA