Establishment of stable expression cell line of canine parvovirus VP2 protein Abstract: Canine parvovirus (CPV) is an acute infectious disease. The main manifestations of this disease are acute hemorrhagic gastroenteritis and acute myocarditis. This disease is highly contagious, with high morbidity and mortality, and it is a harmful dog. ....
Isolation, identification, sequence analysis and fusion expression of VP2 gene of canine parvovirus Abstract: Canine parvovirus belongs to parvovirus subfamily, which is a self-replicating parvovirus family. Canine parvovirus (CPV) is an important disease in dogs. ....
Detection of canine parvovirus by PCR Abstract: Canine parvovirus (CPV) can cause hemorrhagic necrotizing enteritis and myocarditis in dogs. Because of its high morbidity and mortality, and rehabilitation dogs may also carry drugs for a long time, it is extremely harmful to experimental dogs, military dogs and police dogs. In recent years, it has attracted domestic and international attention. ....
Isolation and identification of canine parvovirus YZ strain, preparation of monoclonal antibody and preliminary application Abstract: The epidemic investigation, autopsy and laboratory diagnosis of canine diarrhea, hematochezia and high mortality in Beagle experimental animal base in Jiangsu Province in winter of 1999 were carried out. HA detected the stool samples of dogs with hemorrhagic enteritis, and the hemagglutination value was as high as 2~( 1 ....
Establishment and preliminary application of joint PCR detection method for canine adenovirus and canine parvovirus Abstract: The homology of CAV and CPV reported in GenBank was compared and analyzed respectively, and the E3 region of CAV conserved sequence and NS 1 region of CPV conserved sequence were selected as amplification regions. Two pairs of suitable combined PCR primers were designed and screened by DNAsis software. ....
Abstract: In this study, the genetic variation and evolution of canine parvovirus CPV isolate in Beijing were detected. According to the published CPV capsid protein VP2 gene sequence and the differences of amino acid sites among CPV-2, CPV-2a and CPV-2b, a new method was established. ....
Molecular biological characteristics of VP2 gene of canine parvovirus Abstract: VP2 gene of canine parvovirus CPV encodes the major capsid protein, which plays an extremely important role in the infection of canine parvovirus. It encodes the major antigenic determinant of CPV and can induce the body to neutralize it. ....
Abstract: In order to study the canine parvovirus gene vaccine, primers were designed according to the published VP2 gene sequence of canine parvovirus (CPV), and two VP2 gene fragments (with and without CpG motif at the 3' end) were amplified from CPV-YZ strain by high-fidelity PCR. ....
Study on the application of monoclonal antibody against canine parvovirus in the treatment of canine parvovirus Abstract: In this experiment, 343 canine parvovirus diseases were treated by intramuscular injection of CPV monoclonal antibody, intravenous injection of canine immunoglobulin and intravenous injection of canine albumin, and satisfactory results were obtained. 1.3 .....