Intestinal microecosystem is an important foundation of mammalian health and diseases, and plays an important role in physiology, pathology, prevention and treatment. The development of modern science and technology, such as endogenous biology, anaerobic culture technology, electron microscope technology, cell and molecular biology, genomics, metabonomics and protein genomics, has played a positive role in promoting the study of intestinal microecology. In Biolog and other research methods, it is necessary to extract the microorganisms in the intestine first, and the obtained bacterial suspension not only ensures the species and quantity of living microorganisms, but also removes impurities that may interfere with the research results.