Growth state: adherent growth
Huiying Bio provides HT-22 cells in the form of cryopreservation or resuscitation, and the frozen cells are delivered in 2-3 working days (sufficient dry ice is prepared in advance), and the resuscitation cycle is about 10 working days.
Shanghai Huiying Bio-provides human cell STR identification service, which is very cheap, 800 yuan/plant, and a large quantity is better, providing specific experimental reports. In addition to the identification of human cells (1 1 locus +9 polymorphic loci recommended by ATCC), it also provides mouse strain identification (SNP method) and mouse cell STR typing identification (9 STR loci +2 personal contamination loci).
Huiying Bio has 10 years of SNP typing experience and has completed nearly 10 million SNP typing data. LDR Technology has published hundreds of SCI papers, three of which were published in the top international journal Nature Genetics. Competitive price in the market! Our company also has SNP typing service; STR typing; Molecular genetics and molecular biology services (SNP/STR typing, CNV detection, sequencing, real-time QCPR, microRNA detection, MCF gene expression analysis system, customized qPCR array).
Cell signal pathway analysis service
Brief introduction of service items
Cell signal transduction refers to the process that extracellular factors combine with receptors (membrane receptors or nuclear receptors) to trigger a series of biochemical reactions and protein interactions in cells until the genes needed for cell physiological reactions begin to express and form various biological effects.
application area
① Regulation (inhibition or enhancement) of cell signal transduction
② Regulation of target protein on known protein
③ promoter/enhancer detection
④ Virus/cell interaction
⑤ Transcription factor analysis
⑥ Drug induction or action
⑦ Study on the Regulation of Cytokines by Various Treatments
service cycle
Our company will deliver the experimental results within 6~8 weeks after officially accepting the experimental entrustment.
Huiying Bio is a perfect product supply channel and after-sales service. We strive for perfect work, and strive to be more professional, faster and more timely in dealing with customer consultation, ordering, arrival, after-sales service and technical support.
Shanghai Huiying Bio's management policy is "quality first, honesty first", taking "quality is the life of an enterprise and honesty is the capital of operation" as a warning, strictly controlling the quality of the goods sold and not doing anything that harms the interests of customers.
Huiying Biology has been providing materials or research conditions for the work of scientific researchers. We rely on the trust of the vast number of researchers to develop so far, and our scientific research work with the vast number of researchers has been growing. We have always been very grateful to many new and old customers, and now it is the peak season of reagent procurement for one year. In order to thank and give back to the old and new customers, we have specially introduced Suer's best-selling animal serum to ensure the authenticity, stable quality and minimum batch difference, which will definitely make you feel something. Sur Bio hopes that scientific research can progress step by step and live a happy life.
Huiying cell bank has 400 cell lines and more than 20 drug-resistant strains, with ultra-low discount. Types include: fibroblasts, long-tailed green monkey embryonic cells, long-tailed green monkey kidney cells, mouse breast cancer cells, human bladder cancer cells, human T-cell leukemia cells, human renal adenocarcinoma cells, human renal clear cell adenocarcinoma cells, human thyroid cancer cells, ovarian cancer cells, colon cancer cells, gastric cancer cells and breast-like cells.
The following are after-sales tips after purchasing cell lines:
1. How to keep the water tray of CO2 incubator clean?
Replace it regularly (at least once every two weeks) with sterile distilled water or sterile deionized water.
2. Why is the color of the medium stored in the refrigerator at 4℃ dark red and the pH value more and more alkaline?
When the culture medium is stored in the refrigerator at 4℃, the CO2 in the culture medium will gradually overflow, resulting in the culture medium becoming more and more alkaline. The color of the acid-base indicator (usually phenol red) in the culture medium will also turn dark red with the increase of alkalinity. Because the medium is alkaline, cell growth will stagnate or die. If the culture medium is alkaline, sterile filtered CO2 can be introduced to adjust the pH value.
3. Are all kinds of culture dishes and flasks the same?
Different brands of Petri dishes or flasks have different polymers and different manufacturing procedures. Although it has little effect on most cells, a few cells may have significant growth differences due to the use of different brands of Petri dishes or flasks.
4. Why are there too few cells after the purchased cell freezing tube is thawed? What are the possible reasons for cell death or poor cell survival rate?
The survival rate of researchers in cell culture is very poor, and the common reasons can be summarized as: improper use of culture medium or poor quality of culture medium. Misuse of serum or poor quality of serum. An error occurred during thawing. After the frozen cells are thawed, they are washed and centrifuged. Suspended cells are mistaken for dead cells. Improper use of culture temperature. The cells were left at -80℃ for too long.
Hippocampal neuron cell line, HT22, is a sub-line derived from parental HT4 cells, which originally came from immortalized cells of primary mouse hippocampal neuron culture.