"The comparison of related genomes has gradually become a powerful tool to interpret genomes. In this research report, we sequenced and compared the genomes of 29 real mammals. We have confirmed that at least 5.5% of the human genome has undergone purification and selection, and the conserved sequences covering about 4.2% of the whole genome have been located. By comparing evolutionary markers with experimental data sets, we point out the possible functions of about 60% conserved sequences. In these sequences, we found some new coding exons, possible stop codon read-through events, and more than 10000 synonymous conserved sequence sites located in the exon region. We found that 220 possible RNA structural families and nearly/kloc-0.0 million sequence elements overlap with potential promoters, enhancers and insulators. We have found specific amino acid residues that have been screened out, and 280 000 noncoding sequences with functional changes (transformed from mobile sequences and more than 1000 sequences accelerated by apes and humans). The overlapping of mutation sequences related to some diseases means that our research results will be beneficial to the study of human biology, health and diseases. "