1, different objects: the screening and identification of microbial gene mutation mainly aims at microbial strains, and the changes of their viability and phenotypic characteristics are observed to determine the mutation; The screening and identification of gene mutation in animals and plants is mainly aimed at individual animals and plants, and the mutation situation is judged by observing their external characteristics, growth and development, physiological metabolism and other changes.

2. Different methods: mutation treatment and physiological and biochemical methods are mainly used to screen and identify microbial gene mutations, focusing on the changes of microbial viability and phenotypic characteristics under different conditions; The screening and identification of gene mutation in animals and plants mainly focus on the changes of individual external characteristics, growth and development, physiological metabolism and so on by observing and analyzing phenotypic variation.