After microorganisms are injected into the oil layer, the growing bacteria and metabolites form sediments containing heavy metals, which plays an efficient plugging role; Bacteria ferment in the oil layer, grow and reproduce, and produce gases such as H2, CO2 and CH4, which can reduce the viscosity of crude oil and increase the formation pressure after being dissolved with crude oil. Biopolymers produced by injecting bacteria into water injection reservoirs can adjust the water absorption profile of water injection reservoirs; Underground fermentation produces biosurfactants to reduce the interfacial tension between oil and water; Organic solvents such as organic acids, organic esters and alcohols can also be generated. , increase reservoir porosity, change rock surface properties, and "release" adsorbed oil, thus improving oil recovery.

For you, the problem is that alkanes and fatty acids have the same chain length, but microorganisms generally degrade heavy hydrocarbons in crude oil and produce surfactants, which can reduce the viscosity of crude oil and thus lower the freezing point.

After long-chain degradation, the freezing point will be significantly reduced.