Microscope specimen making can be divided into six steps for beginners' reference:

1. Take a slide and a cover glass and clean them with a soft cloth. Because the cover is very thin, be careful when wiping. You can pinch the edge of the cover glass with your left thumb and forefinger, spread the gauze on the palm of your right hand, gently hold the cover glass from the upper and lower sides, and gently wipe it slowly and evenly, so as not to break the cover glass.

2. Absorb 1~2 drops of clean water with a dropper and put it in the center of the slide.

3. Tear off a small piece of the observed object with tweezers (whether observing cell tissues or organs, the material must be made into thin slices before observation. These sheets should not be too thick. Generally, the thickness of a layer of cells is better. If it is too thick, not only the cells overlap each other, but also the light is not easy to penetrate. Although the outline can barely be seen under the microscope, the detailed structure is difficult to see), put it on the glass slide into small drops, and try not to let the material shrink.

4. Take a clean cover glass with tweezers, first contact one side of the cover glass obliquely with small water drops, then slowly put down the cover glass and cover all the observation materials. When operating, prevent the cover glass from falling suddenly, so as to avoid bubbles and hinder the observation effect.

5. The prepared glass slide requires that the space between the cover glass and the glass slide is just filled with water; When the water is insufficient, a small amount of water can be dripped from the edge of one side of the cover glass with a dropper, and the other side is sucked with absorbent paper, so that the space between the cover glass and the slide glass is filled with water; When there is excess water, absorbent paper can be used to absorb the excess water.

6. Dyeing: a little iodine solution is dripped from the edge of one side of the cover glass with a dropper, and the other side is sucked with absorbent paper, so that the gap between the cover glass and the glass slide is filled with iodine solution; When the iodine solution is redundant, absorbent paper can be used to absorb the excess iodine solution. After the production is completed, it can be observed under a microscope.