In view of the high heterozygosity of pueraria lobata, the researchers chose PacBio and Hi-C sequencing. The genome size of pueraria lobata is 1.38Gb, Contig N50=598 kb, and 99.3% of the sequences are anchored on 1 1 chromosome. BUSCO estimated that the genome integrity was 92.9%. Through annotation, * * * obtained 45,270 protein coding genes, 94.4% of which can be annotated functionally, and the proportion of repetitive sequences in the genome is 62.7%.
Comparative genomic analysis of pueraria lobata and its 16 related species (including 5 leguminous plants) showed that:
By analyzing the transcriptome and metabonomics of high puerarin ZG- 19 and low puerarin ZG-39, the researchers detected 6 14 kinds of 225 differential metabolites (DMs) and 18 14 kinds of differentially expressed genes (DEG), and the rich functional categories of DMs and DEG overlapped with flavonoids and isoflavones.
The correlation coefficient between metabolites and gene expression was further analyzed. The results showed that there was a high correlation between metabolites and gene pairs in the samples, and 60% significant correlation involved up-regulated metabolites and down-regulated or unchanged genes. In the significant correlation of 15%, the metabolic products and gene expression changed in the same direction.
In addition, researchers found a large number of DMs and DEG in isoflavone biosynthesis pathway. This fully analyzed the biosynthetic pathway of pueraria isoflavones.
Through homologous gene search, the researchers found that nine gene families encoding key enzymes of puerarin synthesis pathway all expanded in pueraria lobata; By analyzing the genes that catalyze glycosylation modification in glycosyltransferase family, * * * identified 104 GT genes, of which 13 genes were homologous to 8-C- glucosyltransferase (8-C-GT) and 6 genes were homologous to PIUGT43 gene that catalyzed the c-glycosylation of daidzein to puerarin.
The gene encoding soybean isoflavone synthase (IFS) (CHR11G3854.1) catalyzes the synthesis of daidzein, an intermediate metabolite in puerarin synthesis, and is identified to be highly related to the synthetic pathway of puerarin. In a word, the above analysis preliminarily analyzed the biosynthetic pathway of puerarin in Pueraria lobata.
To sum up, this study analyzed the evolutionary characteristics of pueraria lobata genome by constructing high-quality pueraria lobata genome; The biosynthetic pathway of isoflavones and puerarin, the important secondary metabolites in Pueraria lobata, was deeply analyzed by multi-group analysis, which provided a new idea for the resource utilization, inheritance and breeding research of Pueraria lobata.
In recent years, Yan's team from the Institute of Economic Crops of Guangxi Academy of Agricultural Sciences has been carrying out joint research with Huazhong Agricultural University, Fraser Gene, Shanghai University, guangxi university of chinese medicine and Guangxi Medical University, and has made a series of achievements in the collection, identification and evaluation of global pueraria resources, pueraria resources classification, pueraria genome and molecular biology, kudzu vine and kudzu vine variety breeding, healthy seedling production, high-yield and efficient cultivation, etc. Up to now, the team has collected 4 19 global pueraria germplasm resources, including pueraria lobata, pueraria lobata, pueraria lobata, pueraria lobata, pueraria lobata, pueraria lobata and pueraria lobata. By developing SSR molecular markers of pueraria lobata, the core germplasm bank of pueraria lobata in Guangxi was constructed. The metabolic differences affecting the edible quality and medicinal quality of pueraria lobata, pueraria lobata and pueraria lobata were analyzed by using widely targeted metabonomics technology. Combined with phenotypic identification, the phylogenetic relationship between pueraria lobata and its related species was revealed through chloroplast genome research. Structural genes and transcription factors related to puerarin biosynthesis and metabolic regulation have been excavated, and the function of related genes is being verified. Breeding new varieties of pueraria lobata suitable for development, such as pueraria lobata with high puerarin content, pueraria lobata without residue and medicinal pueraria lobata, and gradually establishing supporting seedling breeding and efficient cultivation techniques. The above research results have been published in Horticultural Research, Frontier of Plant Science, Molecular, Journal of Plant Genetic Resources and Journal of Plant Physiology. The related research was supported by the National Natural Science Foundation of China and Guangxi Science and Technology Department. The publication of pueraria lobata genome article will further promote the evolution and classification research of pueraria plants around the world, promote the scientific and technological progress of pueraria lobata industry in China, play the role of source supply of basic research, and further promote the high-quality development of pueraria lobata industry with local characteristics and advantages in Guangxi.
Speaking of pueraria lobata, everyone must be familiar with it. Pueraria lobata was first used for ecological management in the United States, and was listed as an invasive organism when it was flooded. Pueraria lobata industry in Thailand and its health care effect are popular all over the world. The literature about Pueraria lobata first appeared in the Zhou Dynasty, and Shennong Herbal Classic recorded that "(Pueraria lobata) can cure thirst, body heat, vomiting, joint pain, Yin Qi and detoxify". Pueraria lobata has the functions of relieving muscle fever, promoting fluid production to quench thirst, penetrating rash, tonifying yang to stop diarrhea, dredging meridians and relieving alcoholism. Modern pharmacological research shows that Pueraria lobata has good pharmacological activities in improving cardiovascular system, antioxidation, lowering blood sugar, relieving fever, anti-inflammation, relieving alcoholism and protecting liver, neuroprotection, anti-osteoporosis and estrogen-like effects.
Pueraria lobata, a leguminous plant, is a kind of medicinal and edible plant. Known as "Asian Ginseng" and "Southern Gebei Ginseng", it is widely planted in Guangxi, Guangdong, Jiangxi, Hunan, Hubei and other places. Among them, Guangxi is the main planting area of Pueraria lobata, and the planting area ranks first in the country! Among them, heping town, tengxian, Wuzhou is the famous "Hometown of Pueraria lobata" in China, and the Gesetianxiang and Heping Pueraria lobata Industry (Core) Demonstration Zone in tengxian was rated as a four-star demonstration zone of modern characteristic agriculture (Core) in Guangxi. At present, the development of pueraria lobata industry in Guangxi still faces many problems to be solved urgently. The analysis of pueraria lobata genome will provide scientific and technological support for the high-quality development of pueraria lobata industry.
From: /s/wJkSj2pVJAQibio3unCMEw