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Outline of medical laboratory practice
Graduation Practice Outline of Medical Laboratory Technology (College)

First, the purpose requirements

Graduation practice is an important stage for students to complete the training objectives stipulated in the teaching plan. Through clinical practice, professional theoretical knowledge can be consolidated and improved, and students can master various basic inspection techniques skillfully, so that they can operate and analyze inspection results independently. Specialized practice requires mastering the basic operation and process of outpatient and emergency medical examination; Master the basic skills and operation of general blood and urine examination; Using the basic knowledge and skills of clinical immunology, mastering all kinds of basic operation techniques of clinical immunology inspection, and being familiar with the structure, principle and application of commonly used immune automation instruments at present, we can analyze the results of inspection items. Initially have the ability to engage in inspection technology work that meets the graduation level of junior college.

Second, the internship content

Clinical (basic) test part:

(A) the basic theory

1. Master the method of specimen collection and the preparation of common reagents for general clinical examination.

To master the items and clinical significance of routine peripheral blood examination.

Master the types and precautions of urine samples; The content and clinical significance of urine test: the items, principles, precautions and clinical significance of urine dry chemical test strip detection.

To master the methodological evaluation and clinical significance of fecal occult blood test.

Master the cleanliness evaluation of vagina-vagina secretion.

2. Familiar with the routine examination items and clinical significance of semen samples.

Familiar with the routine examination items and clinical significance of cerebrospinal fluid and serous cavity specimens

3. Understand the quality control process of blood analyzer.

Understand the methods of exfoliative cytology; Characteristics of exfoliated cells in each system.

(2) Basic skills

Immunological examination part:

(A) the basic theory

1. Master commonly used serological detection techniques: determination of serum markers of hepatitis B, rheumatoid factor, circulating immune complex and immunoglobulin.

Master the commonly used enzyme-linked immunosorbent assay technology, the use of enzyme-labeled instrument and the use of washing machine.

2. Familiar with gel diffusion testing technology.

Familiar with the commonly used immune marker detection techniques: luminescence immunoassay, fluorescence immunoassay, dot immunogold permeation test of urine β-HCG, dot immunochromatography test of Chlamydia trachomatis.

3. Understand the radioimmunoassay technology.

Understand the immunofixation test technology.

Understand the reaction technology of complement and complement participation.

(2) Basic skills

Third, the specific arrangements for the internship

College students majoring in medical laboratory can contact themselves or be assigned by the learning center to hospitals and medical laboratory centers (hospitals and institutes) that meet the internship requirements for clinical laboratory and immune laboratory, and each internship lasts for six weeks.

When students enter the practice department for clinical practice, they must attend the entrance education and listen to the teacher's introduction to the management mode and daily work system of the department. Under the guidance of the teaching teacher, be familiar with the work flow and master the operating norms of the department. Without the teacher's consent, you are not allowed to collect specimens and conduct various technical operations without authorization.

During the internship, students should obey the arrangement of their departments, participate in the work of the departments on duty, complete a certain amount of inspection tasks, and make work records. The inspection results obtained in the work must be reviewed and signed by the teaching teacher, and students are not allowed to issue inspection reports without authorization.

Students should take good care of equipment and valuable instruments. It must be used under the guidance of a teacher, and unauthorized use is not allowed. Every day after the experiment, we should take the initiative to clean, tidy and disinfect the laboratory, and tidy up the workbench and all instruments in time before leaving.

During the internship, students should actively participate in various business lectures and academic reports, and carefully take internship notes. And under the guidance of the teaching teacher, combined with the actual work, collect and sort out a study material of medical examination.

At the end of each round, the head of the internship department and the teaching teacher are responsible for giving the students an exam to assess the theoretical knowledge and basic skills that the students should master. I will write a written summary and the teaching teacher will identify the interns.

Graduation practice program for medical laboratory specialty (undergraduate and junior college)

First, the purpose requirements

Graduation practice is an important stage to complete the training objectives stipulated in the teaching plan. Through practice, students can closely connect theoretical knowledge with clinical practice, cultivate students' habit of active learning and independent thinking, master various basic inspection techniques skillfully, and have the ability to operate independently, analyze inspection results and conduct preliminary scientific research. Undergraduate internship requires mastering the basic operation techniques and inspection methods of microbial inspection on the basis of mastering the clinical inspection operations of outpatient and emergency departments; Master the basic skills and operation of general blood examination; Master the characteristics of hemogram and bone marrow picture of common blood diseases, as well as the related laboratory tests of hemolytic diseases and hemorrhagic coagulation diseases, and can use the knowledge learned to analyze and judge the test results with practice. Master scientific research methods such as medical information retrieval, clinical data collection and analysis, and reach the level of laboratory professionals who graduated from university.

Second, the internship content

Clinical (basic) test part:

(A) the basic theory

(1) Master the method of specimen collection and the preparation of common reagents in general clinical tests.

Master the types and precautions of urine sample collection; The content and clinical significance of urine test: the items, principles, precautions and clinical significance of urine dry chemical test strip detection.

To master the methodological evaluation and clinical significance of fecal occult blood test.

Master the cleanliness evaluation of vagina-vagina secretion.

(2) Be familiar with the routine inspection items of semen samples.

Familiar with the routine examination items of cerebrospinal fluid and serous cavity specimens.

(3) Understand the methods of exfoliative cytology; Characteristics of exfoliated cells in each system.

(2) Basic skills

Clinical hematology test part:

(A) the basic theory

(1) To master the routine examination items of peripheral blood and its clinical significance.

Master the examination of hemolytic diseases: incubation fragility test, free hemoglobin determination and abnormal red blood cell morphology analysis.

Master the examination of hemorrhagic diseases: determination of PT, APTT, Fg, TT and procoagulant activity of coagulation factors.

Master the experimental examination of DIC: PLG determination, FDP determination and D- dimer determination.

(2) Familiar with the morphological classification of bone marrow cells under Wright's staining.

Familiar with the operation process of blood analyzer.

Familiar with the principle and clinical application of histochemical staining (peroxidase staining, glycogen staining, alkaline phosphatase staining, iron staining).

Familiar with platelet function test: platelet antibody test and platelet aggregation test.

(3) Understand the quality control process of blood analyzer.

To understand the items and significance of prethrombotic state and molecular markers: TM, P selectin, F 1+2, TAT, FPA.

(2) Basic skills

Microbiological examination part:

(A) the basic theory

(1) Master the collection, handling and inoculation of the following common clinical specimens: blood, cerebrospinal fluid, urine, feces, sputum, puncture fluid and reproductive tract secretions.

Master the common bacterial staining methods-Gram staining and acid-fast staining.

Master the morphology, size, arrangement and dyeing of various bacteria.

Proficient in ink dyeing and lactic acid phenol indigo dyeing.

Master the culture and separation techniques of common clinical pathogens.

Master the identification technology of common clinical pathogens.

Master the microscopic examination, culture and identification of Mycobacterium tuberculosis smear.

(2) Be familiar with the types of common dyeing solutions and culture media.

Familiar with the culture and identification techniques of common clinical anaerobic bacteria.

Familiar with the culture and identification of mycoplasma.

Familiar with the separation, culture and identification of Candida albicans and Cryptococcus neoformans.

Familiar with drug sensitivity test in vitro.

(3) Understand the isolation and culture of virus and the application of PCR technology in microbial detection.

Understand the relevant knowledge of biosafety management in microbiology laboratory.

(2) Basic skills

Third, the specific arrangements for the internship

Undergraduate students majoring in medical laboratory can contact themselves or be assigned by the learning center, but they must go to the second-class hospitals and medical laboratory centers (hospitals and institutes) that meet the internship requirements. On the basis of consolidating the basic skills of clinical laboratory, they will arrange six-week clinical laboratory (mainly hematological examination) and microbiological examination to improve the knowledge and skills of clinical laboratory in professional fields.

During the internship, students obey the arrangement of the department, work under the guidance of the teaching teacher, abide by the work flow and the operating specifications of the department, attend the duty of the department, complete a certain amount of inspection tasks, keep a good record of their work, do not use expensive instruments and equipment, collect specimens and issue inspection results reports without authorization, which meets the requirements of junior college students.

At the end of each round, the head of the internship department and the teaching teacher are responsible for giving the students an exam to assess the theoretical knowledge and basic skills that the students should master. I will write a written summary and the teaching teacher will identify the interns.

As soon as students enter the internship unit, they should contact and hire a graduation thesis writing instructor. The instructor can be the same person as the intern teacher, or can be hired separately, but generally it should be a professional with technical secondary school education or above, or an intermediate professional title inspection professional with postgraduate education. During the writing of graduation thesis, students should keep close contact with their tutors and follow their instructions. The format of graduation thesis is generally a thesis, and it can also be a summary when not applying for a degree. Graduation thesis writing starts from entering the internship unit and lasts for 20 weeks.