Commonly used are gas chromatography and thin layer chromatography. According to the principle that petroleum ether can extract free fatty acids from tea, the extracted free fatty acids were methylated and separated and detected by gas chromatography. The latter was extracted with chloroform-methanol and concentrated, and then the sample was spotted on silica gel G thin plate, and the spectrum was expanded with the mixed solution of n-hexane, ether and formic acid. After color development, it can be detected by thin-layer scanner.
Free fatty acids, also called nonesterified fatty acids NEFA, have little content in serum. If a small amount of serum samples are used for determination, sensitive methods must be adopted, and the interference of fatty acids produced by fat hydrolysis should be avoided.
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Direct heat source: Free fatty acids are substances decomposed by neutral fats. When liver sugar, the energy needed for muscle activity, is exhausted, adipose tissue will decompose neutral fat into free fatty acids for energy use. Therefore, free fatty acids can be said to be substances needed for lasting activities. Like running a marathon.
Clinically, fatty acids with more than 10(c 10) are called free fatty acids (FFA), which are mainly released into the blood by the decomposition of glycerides stored in adipose tissue.
FFA is a lipid that can directly participate in metabolism in blood and is absorbed and utilized by skeletal muscle, myocardium, brain and other tissues. FFA is the energy source and the most active metabolite of human body, and also participates in cell proliferation, inflammatory reaction, hormone regulation and so on.