abstract
The content of neutral detergent fiber (NDF) in double-low rapeseed skin was studied.
Set indicators. Mixed nested design was used to separate dry NaOH method (treatment time was d 1, d2, D 3) and urea.
Ammonification method (treatment time is 1d0, 1d5, ZOd), alkalization-ammoniation compound treatment (treatment time is
15d, ZOd, 25d) to screen out the best treatment group and its treatment time; L (3 ′) orthogonal design was used to select the best technological conditions.
Cellulase, β-glucanase, xylanase and α -amylase were better in the treatment group. So as to obtain the best chemical properties.
Treatment group and the best compound enzyme preparation treatment group. Scanning electron microscope (SacunnigEleetrno)
Scanning electron microscope (SEM) was used to analyze the structure of double-low rapeseed skin treated by the above-mentioned optimal treatment group. And individually
Straw, untreated double-low rapeseed skin, best chemically treated double-low rapeseed skin and best compound enzyme preparation group.
The effects of different diets on the growth performance of Macheng black goat in Hubei Province were studied by using the treated double-low rapeseed skin as coarse material.
The ring. The test results are as follows:
Compared with the control group (untreated group), the double-low rapeseed skin was treated by dry Na0H method, urea ammoniation method and alkalization method respectively.
After ammoniation, the content of neutral detergent fiber (NDF) decreased and the degradation rate of neutral detergent fiber (2h4) increased.
The content of neutral detergent fiber (NDF) in double-low rapeseed skins treated by different chemical methods was significantly different among groups.
p(& lt； 0
.
0 1), the 2h4 degradation rate of neutral detergent fiber was significantly different between groups (P
After the same method and different time treatment, the influence of time on NDF content and 24h degradation rate of neutral washing fiber is very great.
Significant (P
They were increased by 74.22%, 66. 16% and 58.64% respectively, and the urea ammoniation treatment group was improved compared with the control group.
63
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8 1%, 19.()7%, Joe. 64%, the combined treatment of alkalization and ammoniation increased by 28.88% compared with the control group.
58
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04%、7.79%。
There was no significant difference in NDF content among groups after double-low rapeseed skins were treated with different proportions of compound enzyme preparations.
p(& gt； There was no significant difference in the degradation rate of neutral detergent fiber 2h4 among groups (P >:0. 005). Compared with the control group,
The content of neutral detergent fiber decreased by 8.65438 0.05%, 6.59%, 8.56%, 9.865438 0%, 8.42% and 9.57% respectively.
seven
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The nitrogen content is 7.62% and 8.05% respectively; The degradation rate of that neutral was fiber in 24 hour is improved by 88.07%,
89,05%、72.75%、6 1.42%、59.29%、54.77%、68.72%、64.96%、90.74%。
Scanning electron microscope observation showed that the physical structure of double-low rapeseed skin changed significantly before and after treatment.
The epidermis structure of seeds is dense and the organelles are clearly visible. After alkalization-ammoniation compound treatment, the soft tissue swells,
Some "pore" structures are formed, and most organelles disintegrate and fall off: after being treated with compound enzyme preparation, they are doubled.
The surface of low rapeseed skin is smooth, the "pore" structure is more regular, and the organelles basically disintegrate and fall off. Indicating double precision
The cell wall of the treated low rapeseed skin is loose, which provides favorable conditions for the penetration of rumen fluid, thus improving the double low
When studying the nutritional value of animals
Utilization efficiency of rapeseed skin.
Compared with the straw group, the feed intake, daily gain and feed of goats in the double low rapeseed skin group treated by the two treatment methods are better.
There was significant difference in meat rate (P 0.005).
However, the feed intake and daily gain of goats increased, and the feed-meat ratio decreased.
The results showed that the alkali-ammoniation compound method was the best for treating double-low rapeseed skin for 20 days at room temperature. Double low
The optimum compound enzyme preparation ratio of rapeseed skin is cellulase 0.38469, B- glucanase 0. 16679 and xylan.
Enzyme 0.20009, α -amylase 0.0 1259 (added according to the ratio of 5009 double low rapeseed skin content). (Cause) to alkalize.
Compound ammoniation treatment and compound enzyme preparation treatment can improve the nutritional value of double-low rapeseed skin, and the feeding effect is the best
The double-low rapeseed skin treated by better treatment group can improve the growth performance of goats.
Key words: double low rapeseed skin; Ammonification; Compound enzyme preparation; Scanning electron microscope; Rumen nylon bag method
catalogue
chinese abstract
English abstract.
1
order ...............................................................................................................................................................................
1
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1 Importance of developing double-low rapeseed skin feed.
Characteristics and nutritional value of 1.2 double-low rapeseed skin.
1
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3 treatment methods of low-quality roughage ........................................................................................................................................................................
1.4 the problem of using low-quality roughage as animal feed 7
1
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5 The purpose and significance of this experimental study ........................................................................................................................................................................
2
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Materials and methods.
2. 1 sampling .....................................................................................................................................................................
2.2 Chemical treatment method of double-low rapeseed skin .....................................................................................................................................................................
2.3 Enzymatic preparation method for treating double-low rapeseed skin
2.4 Determination of rumen degradation rate of nutrients in double-low rapeseed skin
2.5 scanning electron microscope analysis ..........................................................
.26 goat feeding experiment ........................................................
2.7 detection indicators .................................................
2,8 Calculation formula and data processing
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Results and analysis.
3, 1, double low content of main components in rapeseed skin ......................................
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2. Composition analysis of chemically treated double-low rapeseed skin. ...................................................................................................................................................................
3.3 Composition analysis of double-low rapeseed skin treated with compound enzyme preparation 17.
3.4 SEM analysis of structural changes of double-low rapeseed skin before and after treatment 20
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5 Effects of different diets on growth performance of goats 23
four
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Discuss,,,,,,,,.
4. 1 Comparison of the effects of different treatments on double-low rapeseed skin 23
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2 Effects of different treatments on the physical structure of double-low rapeseed skin
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3 Effects of different diets on growth performance of goats
conclusion
references
thank you ................................................................................................................................................................................
Appendix ... Annex.
Master's degree thesis