The emergence of SARS- CoV coronavirus indicates that the global cross-species spread of severe respiratory diseases has entered a new era, and it has spread rapidly around the world, which has had a huge economic impact.

Since then, several virus strains have appeared in animal populations, including influenza A virus strains H5N 1, H 1N 1, H7N9 and MERS coronavirus, which have caused considerable diseases, deaths and economic difficulties in epidemic areas.

Although public health measures can prevent the outbreak of SARS coronavirus, recent subgenomics studies have identified virus sequences closely related to SARS that spread in bat populations in China, and these viruses may pose a threat in the future. However, sequence data itself provides the least insight for identifying and preparing future pre-disk viruses.

Therefore, in order to study the possibility of bat coronavirus transmission (that is, the possibility of infecting humans), we constructed a chimeric virus encoding human and animal coronavirus spines from the RsSHC0 14- coronavirus sequence isolated from China horseshoe bat, taking the spine adapted by SARS coronavirus mice as the background.

This hybrid virus enables us to evaluate the ability of this new spinoprotein to cause diseases without relying on other necessary adaptive mutations on its natural basis. Using this method, we identified the coronavirus infection caused by SHC0 14 spinosin intervention in primary human tracheal cells and in vivo, and tested the efficacy of existing immunotherapy on SHC0 14- coronavirus.

In a word, the strategy is transformed into metagenomics data to help predict and prepare for future emergency viruses.