(1) Species and varieties: It is difficult for fruit trees and landscaping trees to produce adventitious roots on branches or adventitious buds on roots. For example, the branches of Shandingzi, Qiuzi Pear, Jujube, Mangosteen, Walnut, Pistacia chinensis, Bare bark tree and Catalpa bungeana have weak ability to regenerate adventitious roots, while the roots have strong ability to regenerate adventitious buds. Therefore, cuttings are not easy to survive, while root cuttings are easy to survive.

Trees belonging to different species have different difficulties in rooting. For example, European grapes and American grapes are easier to root than wild grapes and round-leaved grapes. There are also differences in the difficulty of rooting among different varieties of the same fruit tree or tree.

(2) Tree age and branch position: Usually, cuttings of young trees are easy to take root, but with the increase of tree age, the rooting rate decreases. Cuttings with young branches are easier to survive than cuttings with old branches, because the young meristem of its cortex has strong vitality and is the main factor for the survival of cuttings.

(3) The nutrients stored in cuttings are closely related to the survival and layering of cuttings. First, carbohydrates have a good effect on rooting. For example, the rooting rate of grape cuttings treated with synthetic tryptophan can be improved, and measures such as girdling or girdling fruit branches can make branches accumulate more nutrients and auxin, which is beneficial to rooting or layering of cuttings.