The outline of the paper can help the author to arrange and adjust the use of necessary materials in an orderly manner according to the needs of the argumentation theme. The following is a sample of the outline of biological papers I collected to share with you.
Topic: Effect of Angiopoietin on the Growth and Alkaline Fibrosis of Melanoma Cells
Angiopoietin was originally isolated as a tumor angiogenic factor according to its angiogenic activity. It is known that angiopoietin plays a nuclear transfer role in vascular endothelial cells, accumulates in nucleolus and stimulates the transcription of rRNA, thus inducing the proliferation of endothelial cells and promoting angiogenesis. At present, the research on angiopoietin mainly focuses on how to induce tumor angiogenesis, and then promote the further growth and deterioration of tumor. In this paper, the role of angiopoietin in the growth of melanoma cells was studied, and the effect of angiopoietin on the expression and function of another angiogenic factor, bFGF, was analyzed. We transformed the angiopoietin gene into human melanoma A375 cells in forward and reverse directions, respectively, and obtained stable cell lines with low and high expression of angiopoietin. We found that cell proliferation was inhibited in cell lines transfected with antisense angiopoietin gene, and cell proliferation induced by bFGF was also inhibited, but the expression level of bFGF was increased. On the contrary, in the cell line transfected with the sense angiopoietin gene, the cell proliferation was improved, and the cell proliferation induced by bFGF was also improved, but the expression level of bFGF decreased. In addition, we analyzed the nuclear transplantation of angiopoietin by immunofluorescence experiment, and found that angiopoietin can be transplanted in A375 cells. Further immunotransmission electron microscope analysis showed that the distribution of angiopoietin in nucleolus was the same as that of rDNA and rRNA, and its role might be related to rDNA. In addition, we found that neomycin, an aminoglycoside antibiotic, can inhibit the nuclear transfer of angiopoietin in A375 cells. Neomycin inhibits cell proliferation by inhibiting the nuclear transfer of angiopoietin. To sum up, our results show that angiopoietin promotes the proliferation of A375 cells through nuclear transplantation and participates in the proliferation induced by bFGF. Therefore, angiopoietin can not only play an important role in the growth of melanoma, but also directly promote the proliferation of tumor cells. In addition, we also found that angiopoietin has a negative regulatory effect on the expression of bFGF. Therefore, targeting angiopoietin may find a new way to treat melanoma.
Chinese abstract 3-4
English abstract 4-8
Chapter 1 Literature Review 8-53
I. Relationship between angiogenic factors and tumors 8-37
(1) Introduction to Angiogenesis 8
(ii) Angiogenesis and Tumors 8-9
(III) Anti-angiogenic tumor therapy 9- 1 1
(IV) introduction of angiogenic factor 1 1-30.
(V) Significance of angiogenic factors in tumor growth, metastasis and prognosis 30-37
Two. Structure and function of angiopoietin and its relationship with tumor 37-45
(a) Structure and properties of angiopoietin 37-38
(2) Biological function of angiopoietin 38
(3) The mechanism of angiopoietin 38-4 1
(d) Angiopoietin and Tumour 4 1-42
(5) Angiopoietin inhibitor 42-45
Three. Structure and function of basic fibroblast growth factor and its relationship with tumor 45-49
(I) Structure and properties of bFGF 45-46
(II) Mechanism of bFGF 46-48
(Biological function of bFGF 48
Basic fibroblast growth factor and tumor 48-49
Four. Research progress of melanoma 49-53
(a) introduction to melanoma 49
(2) Melanoma and Angiogenesis Factor 49-5 1
(III) The expression of angiopoietin in melanoma and its role 5 1
(The expression and function of bFGF in melanoma 5 1
(5) Anti-angiogenic factor melanoma therapy 5 1-53
Chapter II Introduction 53-56
I. Research Background and Basis of Proposal 53-55
Second, the research content and significance of this paper 55-56
Chapter III Experimental Materials and Methods 56-70
I. Experimental Materials 56-57
(I) Cells and Plasmids 56
(2) Main reagent 56
(3) Main instruments 56-57
Two. Experimental method 57-70
(i) Mass preparation method of plasmids 57-58
(II) Mammalian cell culture 58-59
(3) mammalian cell transfection 59
(4) Extraction of mammalian cell genome 59-60.
(5) extracting total RNA from mammalian cells.
(6) Reverse transcription polymerase chain reaction (RT-PCR)62-63
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