1. First, add the reaction solution into the reaction tank, add the antigen to be detected into the reaction solution, and stir evenly.

2. Secondly, washing liquid is added into the reaction tank to combine the antigen and antibody in the reaction liquid to form an antigen-antibody complex.

3. Then, a detection signal is added into the reaction tank to make the antigen-antibody complex fluoresce, so as to detect the existence of antigen.

4. Finally, the concentration of antigen is calculated by fluorescence intensity, so as to complete the detection. The above is the application method of fluorescence immunochromatography in Huada Yuan Yin.